| Sulfiredoxin is critical for redox balance and survival of cells exposed to low steady-state levels of H2O2. | |
| | |
MedLine Citation:
|
PMID: 22086924 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Sulfiredoxin (Srx) is an enzyme that catalyzes the reduction of cysteine sulfinic acid of hyperoxidized peroxiredoxins (Prxs). Having high affinity toward H(2)O(2), 2-Cys Prxs can efficiently reduce H(2)O(2) at low concentration. We previously showed that Prx I is hyperoxidized at a rate of 0.072% per turnover even in the presence of low steady-state levels of H(2)O(2). Here we examine the novel role of Srx in cells exposed to low steady-state levels of H(2)O(2), which can be achieved by using glucose oxidase. Exposure of low steady-state levels of H(2)O(2) (10~20 μM) to A549 or wild-type mouse embryonic fibroblast (MEF) cells does not lead to any significant change in oxidative injury because of the maintenance of balance between H(2)O(2) production and elimination. In contrast, loss-of-function studies using Srx-depleted A549 and Srx(-/-) MEF cells demonstrate a dramatic increase in extra- and intracellular H(2)O(2), sulfinic 2-Cys Prxs, and apoptosis. Concomitant with hyperoxidation of mitochondrial Prx III, Srx-depleted cells show an activation of mitochondria-mediated apoptotic pathways including mitochondria membrane potential collapse, cytochrome C release, and caspase activation. Furthermore, adenoviral re-expression of Srx in Srx-depleted A549 or Srx(-/-) MEF cells promotes the reactivation of sulfinic 2-Cys Prxs and results in cellular resistance to apoptosis, with enhanced removal of H(2)O(2). These results indicate that Srx functions as a novel component to maintain the balance between H(2)O(2) production and elimination and then protects cells from apoptosis even in the presence of low steady-state levels of H(2)O(2). |
| | |
Authors:
|
Jin Young Baek; Sun Hee Han; Su Haeng Sung; Hye Eun Lee; Yu-Mi Kim; You Hyun Noh; Soo Han Bae; Sue Goo Rhee; Tong-Shin Chang |
Related Documents
:
|
18539774 - Kinetics of salicylate-mediated suppression of jasmonate signaling reveal a role for re... 15650234 - Molecular mechanisms of fenretinide-induced apoptosis of neuroblastoma cells. 10969794 - Sphingosine enhances apoptosis of radiation-resistant prostate cancer cells. 10321684 - The yins and yangs of ceramide. 19039924 - Modulation of apoptosis of mononuclear cells under conditions of oxidative stress. 15451054 - T cell receptor stimulation, reactive oxygen species, and cell signaling. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-11-15 |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: - ISSN: 1083-351X ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-11-16 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Ewha Womans University, Korea, Republic of. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Angiotensin II increases activity of the Epithelial Na+ Channel (ENaC) in the distal nephron additiv...
Next Document: Wogonin and related natural flavones overcome TRAIL-resistance of tumors by down-regulation of c-FLI...