| Sulfhydryl group quantitation of hepatoma and liver microsomal fractions. | |
| | |
MedLine Citation:
|
PMID: 165877 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The relationship of sulfhydryl and disulfide groups to protein synthesis in normal and rapidly growing tissues was investigated by quantitation of sulfhydryl groups in endoplasmic reticulum and polyribosomes of normal liver and hepatomas. Stripping by ethylenediaminetetraacetate and potassium chloride of normal liver smooth and rough endoplasmic reticulum reduced by 15 percent and increased 30 percent, respectively, the sulfhydryl groups available for carboxamidemethylation by iodoacetamide. This could reflect the removal of ribosomes from rough endoplasmic reticulum with the subsequent exposure of sulfhydryl groups. Exposed sulfhydryl groups of normal mature female rat liver smooth endoplasmic reticulum were decreased to a similar degree by the stripping procedure with ethylenediaminetetra-acetate and potassium chloride when quantitated by either iodoacetamide or 4,4'-dithiodipyridine. This was not the case in young male and female rats, where the stripping procedure failed to decrease the exposed sulfhydryl groups of smooth endoplasmic reticulum. An increase in the quantity of exposed sulfhydryl groups of normal young and mature rat liver rough endoplasmic reticulum after stripping by ethylenediaminetetraacetate and potassium chloride was observed with iodoacetamide. However, when 4,4'-dithiodipyridine was used, no change could be detected. The hypothesis that smooth endoplasmic reticulum arises by degranulation of the rough endoplasmic reticulum in vivo is not supported by our sulfhydryl group quantitation of smooth endoplasmic reticulum and in vitro degranulated rough endoplasmic reticulum. A negative correlation between exposed sulfhydryl groups on the polyribosomes and the rate of growth of normal liver and of Morris hepatomas 6 and 38B suggests that the conformation of the free polyribosomal proteins could be a control factor for the rate of protein synthesis. Faster growing hepatomas also have greater quantities of sulfhydryls and disulfides. |
| | |
Authors:
|
F W Stratman; A A Hochberg; R N Zahlten; H P Morris |
Related Documents
:
|
4606167 - Acceptor activity of hypermethylated e. coli trnaf-met. 10629087 - In vivo electroporetic transfer of bcl-2 antisense oligonucleotide inhibits the develop... 283267 - Glycosphingolipids of subcellular fractions from normal rat liver and morris hepatoma 5... 177287 - Gangliosides of hepatoma 27, normal and regenerating rat liver. 7994187 - Evaluation and comparison of urinary pyridinium crosslinks in two rat models of bone lo... 7872657 - Resistance to oxidation of native lipoproteins and erythrocyte membrane lipids in rats ... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Cancer research Volume: 35 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1975 Jun |
Date Detail:
|
Created Date: 1975-09-04 Completed Date: 1975-09-04 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1476-84 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Age Factors Animals Carcinoma, Hepatocellular / metabolism* Cell Fractionation Disulfides / metabolism Edetic Acid Endoplasmic Reticulum / metabolism Female Iodamide Liver / ultrastructure Liver Neoplasms / metabolism* Male Methylation Microsomes, Liver / metabolism* Neoplasms, Experimental / metabolism Polyribosomes / metabolism Potassium Chloride Protein Binding Protein Conformation Pyridines Rats Sex Factors Sulfhydryl Compounds / analysis* Sulfhydryl Reagents Urea |
| Chemical | |
Reg. No./Substance:
|
0/Disulfides; 0/Pyridines; 0/Sulfhydryl Compounds; 0/Sulfhydryl Reagents; 440-58-4/Iodamide; 57-13-6/Urea; 60-00-4/Edetic Acid; 7447-40-7/Potassium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Nucleolar phosphoproteins of normal rat liver and Novikoff hepatoma ascites cells.
Next Document: Regulation of growth of mouse mastocytoma cells.