Document Detail


Sulfation of endothelial mucin by corneal keratan N-acetylglucosamine 6-O-sulfotransferase (GST-4beta).
MedLine Citation:
PMID:  11352640     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intestinal N-acetylglucosamine 6-O-sulfotransferase (I-GlcNAc6ST, GST-4alpha) and corneal N-acetylglucosamine 6-O-sulfotransferases (C-GlcNAc6ST, GST-4beta) are two highly homologous GlcNAc 6-O-sulfotransferase isozymes encoded by two intronless open reading frames that reside approximately 50 kb apart on human chromosome 16q23.1. I-GlcNAc6ST has been shown to catalyze 6-O-sulfation of the endothelial mucin GlyCAM-1. C-GlcNAc6ST catalyzes 6-O-sulfation of GlcNAc in keratan sulfate and null-mutations in its encoding gene cause human macular corneal dystrophy. We show here that C-GlcNAc6ST efficiently catalyzes sulfation of GlyCAM-1 when coexpressed with the latter in COS-7 cells. We have further compared expression in human of both enzymes by Northern analysis with isozyme-specific probes. While I-GlcNAc6T is expressed mostly in intestinal tissue, larger C-GlcNAc6ST transcripts are found predominantly in the brain.
Authors:
A Bartes; S Bhakta; S Hemmerich
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  282     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-05-15     Completed Date:  2001-06-07     Revised Date:  2007-10-30    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  928-33     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Affiliation:
Department of Respiratory Diseases, Roche Bioscience, 3401 Hillview Avenue, Palo Alto, California 94304, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Brain / metabolism
COS Cells
Cornea / enzymology*
Endothelium / metabolism
Humans
Intestines / metabolism
Mice
Molecular Sequence Data
Mucins / metabolism*
RNA, Messenger / biosynthesis
Sequence Homology, Amino Acid
Sulfotransferases / biosynthesis,  genetics,  metabolism*
Tissue Distribution
Transfection
Chemical
Reg. No./Substance:
0/Mucins; 0/RNA, Messenger; 145895-89-2/sulfated glycoprotein p50; EC 2.8.2.-/Sulfotransferases; EC 2.8.2.-/carbohydrate sulfotransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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