Document Detail


Sulfate detection in glycoprotein-derived oligosaccharides by artificial neural network analysis of Fourier-transform infrared spectra.
MedLine Citation:
PMID:  7978246     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report the use of an artificial neural network to analyze the fingerprint region of Fourier-transform infrared (ir) spectra of oligosaccharides for the presence of sulfate groups. This assay can rapidly and nondestructively detect the presence of sulfate in as little as 1 nmol (approximately 2 micrograms) of a glycoprotein-derived monosulfated decasaccharide. The neural network was trained to recognize the presence of sulfate groups by presenting it with 45 ir spectra of sulfated and nonsulfated mono- and oligosaccharides. No prior knowledge of the characteristic ir spectral features of a sulfate group was needed as input. The training process required between 3 and 10 h, while analysis of a spectrum with the trained neural network requires only 0.1 s.
Authors:
D A Powell; V Turula; J A de Haseth; H van Halbeek; B Meyer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical biochemistry     Volume:  220     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-12-12     Completed Date:  1994-12-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  20-7     Citation Subset:  IM    
Affiliation:
Complex Carbohydrate Research Center, University of Georgia, Athens 30602-4712.
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MeSH Terms
Descriptor/Qualifier:
Carbohydrate Sequence
Glycoproteins / chemistry*
Molecular Sequence Data
Neural Networks (Computer)*
Oligosaccharides / chemistry*
Spectroscopy, Fourier Transform Infrared
Sulfuric Acid Esters / analysis*
Grant Support
ID/Acronym/Agency:
P41-RR-05351/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Glycoproteins; 0/Oligosaccharides; 0/Sulfuric Acid Esters

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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