Document Detail


Suitability of polyelectrolyte shells modified with fullerene derivate for immunoisolation of cells. Experimental study.
MedLine Citation:
PMID:  23029999     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
The polymeric permiselective membranes application for immunoisolation of cells separating the transplanted cells from the host immunological system may eliminate immunosuppressive therapy during transplantation. The suitability of polyelectrolyte modified nanocoatings for immunoisolation of cells was assessed. The polymeric shells modified with incorporated fullerene derivate were applied for encapsulation of human T-lymphocyte cell line Jurkat or rat pancreatic islets of Langerhans using layer-by-layer technique. Hydroxylated fullerene was incorporated to the polyelectrolyte shell for hydrophility increase as well as for layer stability improvement. Evaluation with AFM, FTIR, fluorescence microscopy confirmed the nanocoating presence on the encapsulated cells. It was observed that polylysine-polyethyleneimine membrane with incorporated fullerenol allowed for encapsulated cells functioning in vitro. Membrane conformation applied for encapsulation of pancreatic rat islets allowed for glucose level decline during xenotransplantation into mice. The elaborated nanocoating may be recommended as the possible alternative to the space consuming microencapsulation for biomedical purposes.
Authors:
M Borkowska; E Godlewska; M Antosiak-Iwańska; J Kinasiewicz; M Strawski; M Szklarczyk; L H Granicka
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical nanotechnology     Volume:  8     ISSN:  1550-7033     ISO Abbreviation:  J Biomed Nanotechnol     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-10-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101230869     Medline TA:  J Biomed Nanotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  912-7     Citation Subset:  IM    
Affiliation:
Nałecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, 02-109 Warsaw, Poland.
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