Document Detail

Suitability of cryoprotectants and impregnation protocols for embryos of Japanese whiting Sillago japonica.
MedLine Citation:
PMID:  18761007     Owner:  NLM     Status:  MEDLINE    
The purpose of this study was to examine the suitability of cryoprotectant agent (CPA) impregnation protocols for the embryos of Japanese whiting (Sillago japonica), a small-sized, easy-to-rear, and prolific marine fish which may constitute a suitable experimental material for the development of cryopreservation methods for fish embryos. Our immediate goals were to assess the toxicity and permeability of various CPAs to whiting embryos of different developmental stages. Exposure of gastrula, somites, tail elongation, and pre-hatching embryos to 10%, 15%, and 20% solutions of propylene glycol (PG), methanol (MeOH), dimethyl sulfoxide (Me2SO), dimethylformamide (DFA), ethylene glycol (EG), and glycerol (Gly) in artificial sea water (ASW; 33 psu) for 20 min revealed that CPA toxicity for whiting embryos increased in the order of PG<Me2SO<DFA<EG<MeOH<Gly. Relative CPA permeability, estimated by proton nuclear magnetic resonance spectroscopy, showed the same trend as toxicity except for Gly, which was highly toxic but showed only moderate permeability. There were no marked differences in CPA tolerance between developmental stages except for a slight decrease in pre-hatching embryos. Stepwise (20% x 5 steps) impregnation with CPA mixtures of 20-25% PG with 10-15% DFA, Me2SO, MeOH, or EG in ASW were well tolerated by the embryos. Overall, the results of toxicity and permeability suggest that PG, MeOH, and EG could be useful for the development of CPA solutions for whiting embryos.
Sk Mustafizur Rahman; Sullip Kumar Majhi; Toru Suzuki; Shingo Matsukawa; Carlos Augusto Strüssmann; Rikuo Takai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-12
Journal Detail:
Title:  Cryobiology     Volume:  57     ISSN:  1090-2392     ISO Abbreviation:  Cryobiology     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-17     Completed Date:  2008-11-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  170-4     Citation Subset:  IM    
Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-Ku, Tokyo 108-8477, Japan.
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MeSH Terms
Cryopreservation* / methods,  veterinary
Cryoprotective Agents / pharmacokinetics*,  toxicity*
Dimethyl Sulfoxide / pharmacokinetics,  toxicity
Dimethylformamide / pharmacokinetics,  toxicity
Embryo, Nonmammalian / drug effects
Embryonic Development / drug effects
Ethylene Glycol / pharmacokinetics,  toxicity
Gastrula / drug effects
Glycerol / pharmacokinetics,  toxicity
Magnetic Resonance Spectroscopy
Methanol / pharmacokinetics,  toxicity
Permeability* / drug effects
Propylene Glycol / pharmacokinetics,  toxicity
Solutions / chemistry
Somites / drug effects
Reg. No./Substance:
0/Cryoprotective Agents; 0/Solutions; 107-21-1/Ethylene Glycol; 56-81-5/Glycerol; 57-55-6/Propylene Glycol; 67-56-1/Methanol; 67-68-5/Dimethyl Sulfoxide; 68-12-2/Dimethylformamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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