Document Detail

Suitability of chemiluminescent enzyme immunoassay for the measurement of blood tacrolimus concentrations in rheumatoid arthritis.
MedLine Citation:
PMID:  21223955     Owner:  NLM     Status:  Publisher    
OBJECTIVES: The aim of this study was to evaluate the suitability of chemiluminescent enzyme immunoassay (CLIA) for the monitoring of whole-blood tacrolimus concentrations in rheumatoid arthritis (RA) patients. DESIGN AND METHODS: Sixty-three RA patients and 47 renal transplant (RT) patients treated with tacrolimus were enrolled. Tacrolimus concentrations in spiked blood and patient blood were measured by CLIA and HPLC-MS/MS. The cross-reactivity in CLIA was evaluated using 13-O-demethylated or 31-O-demethylated tacrolimus. RESULTS: Tacrolimus concentrations measured by CLIA correlated with those measured by HPLC-MS/MS. Bland-Altman analysis revealed the 95% confidence intervals between CLIA and HPLC-MS/MS in RA and RT patients were -20.7 to 109.9% and -5.0 to 74.1%, respectively. While 31-O-demethylated tacrolimus cross-reaction amounted to an equivalent of 120% tacrolimus in CLIA, 13-O-demethylated tacrolimus did not cross-react. CONCLUSION: CLIA values should be carefully interpreted in RA patients, especially those receiving a low dose of tacrolimus.
Kumi Hirano; Shuji Maruyama; Yasuaki Mino; Takafumi Naito; Junichi Kawakami
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-1-9
Journal Detail:
Title:  Clinical biochemistry     Volume:  -     ISSN:  1873-2933     ISO Abbreviation:  -     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-1-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0133660     Medline TA:  Clin Biochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Inc.
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