| Suitability of Soxhlet Extraction to Quantify Microalgal Fatty Acids as Determined by Comparison with In Situ Transesterification. | |
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MedLine Citation:
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PMID: 22057577 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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To assess Soxhlet extraction as a method for quantifying fatty acids (FA) of microalgae, crude lipid, FA content from Soxhlet extracts and FA content from in situ transesterification (ISTE) were compared. In most cases, gravimetric lipid content was considerably greater (up to sevenfold) than the FA content of the crude lipid extract. FA content from Soxhlet lipid extraction and ISTE were similar in 12/18 samples, whereas in 6/18 samples, total FA content from Soxhlet extraction was less than the ISTE procedure. Re-extraction of residual biomass from Soxhlet extraction with ISTE liberated a quantity of FA equivalent to this discrepancy. Employing acid hydrolysis before Soxhlet extraction yielded FA content roughly equivalent to ISTE, indicating that acidic conditions of ISTE are responsible for this observed greater recovery of FA. While crude lipid derived from Soxhlet extraction was not a useful proxy for FA content for the species tested, it is effective in most strains at extracting total saponifiable lipid. Lipid class analysis showed the source of FA was primarily polar lipids in most samples (12/18 lipid extracts contained <5% TAG), even in cases where total FA content was high (>15%). This investigation confirms the usefulness of ISTE, reveals limitations of gravimetric methods for projecting biodiesel potential of microalgae, and reinforces the need for intelligent screening using both FA and lipid class analysis. |
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Authors:
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Jesse McNichol; Karen M Macdougall; Jeremy E Melanson; Patrick J McGinn |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-6 |
Journal Detail:
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Title: Lipids Volume: - ISSN: 1558-9307 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Institute for Marine Biosciences, National Research Council of Canada, Halifax, NS, B3H 3Z1, Canada, McNichol@mit.edu. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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