Document Detail


Suitability of Commercial Transport Media for Biological Pathogens under Nonideal Conditions.
MedLine Citation:
PMID:  22121364     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
There is extensive data to support the use of commercial transport media as a stabilizer for known clinical samples; however, there is little information to support their use outside of controlled conditions specified by the manufacturer. Furthermore, there is no data to determine the suitability of said media for biological pathogens, specifically those of interest to the US military. This study evaluates commercial off-the-shelf (COTS) transport media based on sample recovery, viability, and quality of nucleic acids and peptides for nonpathogenic strains of Bacillus anthracis, Yersinia pestis, and Venezuelan equine encephalitis virus, in addition to ricin toxin. Samples were stored in COTS, PBST, or no media at various temperatures over an extended test period. The results demonstrate that COTS media, although sufficient for the preservation of nucleic acid and proteinaceous material, are not capable of maintaining an accurate representation of biothreat agents at the time of collection.
Authors:
Kyle Hubbard; Gregory Pellar; Peter Emanuel
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Publication Detail:
Type:  Journal Article     Date:  2011-10-30
Journal Detail:
Title:  International journal of microbiology     Volume:  2011     ISSN:  1687-9198     ISO Abbreviation:  Int J Microbiol     Publication Date:  2011  
Date Detail:
Created Date:  2011-11-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101516125     Medline TA:  Int J Microbiol     Country:  Egypt    
Other Details:
Languages:  eng     Pagination:  463096     Citation Subset:  -    
Affiliation:
Excet, Inc., 8001 Braddock Rd Suite 303, Springfield, VA 22151, USA.
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