Document Detail

Suillus collinitus methanolic extract increases p53 expression and causes cell cycle arrest and apoptosis in a breast cancer cell line.
MedLine Citation:
PMID:  22868134     Owner:  NLM     Status:  Publisher    
In the present work, methanolic, ethanolic and boiled water extracts of Suillus collinitus were chemically characterised and submitted to an evaluation of their bioactive properties (antioxidant potential and cytotoxic activity in tumor cell lines). Phenolic acids and sugars were identified chromatographically and quantified in the methanolic and boiled water extracts, respectively. S. collinitus ethanolic extract had the highest antioxidant activity. Nevertheless, with respect to cell growth inhibition, the methanolic extract was the most potent extract, particularly in MCF-7 cells (GI(50) 25.2±0.2μg/ml). Moreover, the GI(50) concentration of this extract induced a G1 cell cycle arrest, with a concomitant decrease in the percentage of cells in the S phase. Furthermore, it caused an increase in the percentage of apoptotic cells, from 6.0±0.2% in untreated cells, to 15.3±2.0% in cells treated with the GI(50) concentration and to 16.3±2.0% in cells treated with 2×GI(50) concentration. In addition, 48h treatment with the GI(50) concentration caused a strong increase in the levels of p53, p21, and cleaved PARP, together with a decrease in Bcl-2 and XIAP. Results indicate that S. collinitus may be a promising source of bioactive compounds. Particularly, its methanolic extract appears to have a p53-mediated effect on the normal cell cycle distribution and apoptosis induction in a human breast tumor cell line.
Josiana A Vaz; Isabel C F R Ferreira; Catarina Tavares; Gabriela M Almeida; Anabela Martins; M Helena Vasconcelos
Related Documents :
1299374 - Ncam and its polysialic acid moiety: a mechanism for pull/push regulation of cell inter...
21181444 - Role of caga-positive helicobacter pylori on cell proliferation, apoptosis, and inflamm...
391574 - Markers for membranous cell components. the 6th international subcellular methology for...
16656304 - Polarity of iaa effect on sieve-tube and xylem regeneration in coleus and tomato stems.
21072884 - Functional involvement of daxx in gp130-mediated cell growth and survival in baf3 cells.
14525774 - T-cell homeostasis in humans with thymic hypoplasia due to chromosome 22q11.2 deletion ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-30
Journal Detail:
Title:  Food chemistry     Volume:  135     ISSN:  0308-8146     ISO Abbreviation:  Food Chem     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-8-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7702639     Medline TA:  Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  596-602     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Cancer Drug Resistance Group, IPATIMUP - Institute of Molecular Pathology and Immunology of the University of Porto, Portugal; CIMO - Mountain Research Centre, School of Agriculture, Polytechnic Institute of Bragança, Campus de Santa Apolónia, Apartado 1172, 5301-855 Bragança, Portugal; CEQUIMED-UP, Center of Medicinal Chemistry, University of Porto, Portugal; Health School, Polytechnic Institute of Bragança, Av. D. Afonso V, 5300-121 Bragança, Portugal; Department of Biological Sciences, Laboratory of Microbiology, Faculty of Pharmacy, University of Porto, Portugal.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Rapid measurement of total acid content (TAC) in vinegar using near infrared spectroscopy based on e...
Next Document:  Quantitative determination of fatty acid compositions in micro-encapsulated fish-oil supplements usi...