| Sugar-specific endocytosis of glycoproteins by Lewis lung carcinoma cells. | |
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MedLine Citation:
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PMID: 6668306 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lewis lung carcinoma cells from tumors, metastasis nodules, or from culture bind fluorescent derivatives of neoglycoproteins containing alpha-D-glucose residues: This binding is competitively inhibited by neoglycoproteins containing alpha-D-glucose, by mannan, and by several other neoglycoproteins. Cell binding and uptake of the fluorescent derivatives of the neoglycoproteins was quantified by lysing the cells with an alkylpolyol (MAC 19 or MAC 18) and measuring the fluorescence intensity of the supernatant. The amount of cell-associated neoglycoprotein was higher at 37 degrees C than at 4 degrees C with LLC from tumor. The binding and uptake were inhibited by glycoconjugates containing alpha-D-glucose. These results suggest the presence of sugar specific receptors in Lewis lung carcinoma cells which are involved in a sugar-specific binding and endocytosis phenomenon. The implication of the existence of a carbohydrate-binding protein on the surface of Lewis lung carcinoma cells are discussed with regard to the in vivo behaviour of these cells, especially in relation to their metastatic properties and to the possibility of using neoglycoproteins as specific carriers of cytotoxic drugs. Hybrid molecules of gelonin and neoglycoprotein containing alpha-D-glucose were used as targetted toxin: The targetted toxin was found to bind to and to enter the intact cells and was 100 times more toxic than free drug. |
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Authors:
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A C Roche; M Barzilay; P Midoux; S Junqua; N Sharon; M Monsigny |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 22 ISSN: 0730-2312 ISO Abbreviation: J. Cell. Biochem. Publication Date: 1983 |
Date Detail:
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Created Date: 1984-04-26 Completed Date: 1984-04-26 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 131-40 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Cells, Cultured Endocytosis* Glycoproteins / metabolism* Lung Neoplasms / metabolism* Mice Mice, Inbred C57BL Plant Proteins / metabolism, pharmacology Protein Binding Ribosome Inactivating Proteins, Type 1 |
| Chemical | |
Reg. No./Substance:
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0/Glycoproteins; 0/Plant Proteins; 0/Ribosome Inactivating Proteins, Type 1; 75037-46-6/GEL protein, Gelonium multiflorum |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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