Document Detail

Sufficient PpIX production for PDT even with short contact time of topically applied 5-ALA in rabbit tongues.
MedLine Citation:
PMID:  17885797     Owner:  NLM     Status:  MEDLINE    
Although effectiveness of photodynamic therapy (PDT) after application of 5-aminolevulinic acid ointment to oral mucosal lesions has been reported, a consensus regarding recontact time of ALA applied to a lesion has been unreached. Hence, we determined the contact time of ALA required for protoporphyrin IX (PpIX) production efficient for full-blown PDT reaction. ALA ointment was topically applied to healthy rabbits' tongues for different periods and then washed out. On the surface of the tongue, 10-min contact of ALA maximized the PpIX-derived fluorescence. PpIX yield in a tissue specimen with 10-min contact of ALA reached 73% of that in a tissue specimen with 240-min contact. Histological observation showed that PpIX accumulation predominated in the basal layer, and the PDT effects were confined in the mucosal epithelium regardless of contact time. These results suggest that 5-aminolevulinic-acid-ointment-mediated PDT with short contact of ALA is potentially applicable for treating tongue epithelial lesions.
Takehiro Oura; Hideo Tanaka; Yuji Morimoto; Kazuma Masumoto; Eiji Kohno; Toru Hirano; Fuminori Katou; Kenji Hashimoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-09-21
Journal Detail:
Title:  Lasers in medical science     Volume:  23     ISSN:  0268-8921     ISO Abbreviation:  Lasers Med Sci     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-26     Completed Date:  2009-01-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8611515     Medline TA:  Lasers Med Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  355-60     Citation Subset:  IM    
Department of Oral and Maxillofacial Surgery, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu, Shizuoka 431-3192, Japan.
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MeSH Terms
Aminolevulinic Acid / analogs & derivatives,  pharmacology*
Epithelium / drug effects*,  radiation effects
Models, Animal
Mouth Mucosa / drug effects*,  radiation effects
Photochemotherapy / methods*
Photosensitizing Agents / pharmacology*
Protoporphyrins / biosynthesis*,  radiation effects
Time Factors
Tongue / drug effects*,  radiation effects
Reg. No./Substance:
0/Photosensitizing Agents; 0/Protoporphyrins; 106-60-5/Aminolevulinic Acid; 553-12-8/protoporphyrin IX

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