Document Detail


Sucrose synthase activity and carbohydrates content in relation to phosphorylation status of Vicia faba root meristems during reactivation from sugar depletion.
MedLine Citation:
PMID:  22770419     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Carbohydrate starvation of Vicia faba root meristems leads to readjustment of carbohydrate metabolism and blocks the cell cycle in two principal control points (PCP1/2). The cell cycle reactivation is possible after sucrose provision, although with a delay of about 12h. During this period, the cells are sensitive to 6-dimethylaminopurine (6-DMAP) and okadaic acid (OA), inhibitors of protein kinases and phosphatases, respectively. The aim of the present study was to investigate whether those inhibitors are involved in inhibition of cell cycle revival through interference with the activities of two sucrose-cleaving enzymes: sucrose synthase (SuSy; EC 2.4.1.13) and invertase (INV; EC 3.2.1.26). In sugar-starved cells, the in situ activity of both enzymes decreased significantly. Following supplementation of root meristems with sugar, INV remained inactive, but SuSy activity increased. Despite the lack of INV activity, glucose was present in meristem cells, but its content was low in cells treated with OA. In the latter case, the size of plastids was reduced, they had less starch, and Golgi structures were affected. In sugar-starved cells, SuSy activity was induced more by exogenous sucrose than by glucose. The sucrose-induced activity was strongly inhibited by OA (less by 6-DMAP) at early stages of regeneration, but not at the stages preceding DNA replication or mitotic activities. The results indicate that prolongation of regeneration and a marked decrease in the number of cells resuming proliferation (observed in previous studies) and resulting from the action of inhibitors, are correlated with the process of SuSy activation at the beginning of regeneration from sugar starvation.
Authors:
Justyna Teresa Polit; Iwona Ciereszko
Related Documents :
432759 - Isolation of hydroxyurea-resistant cho cells with altered levels of ribonucleotide redu...
2439199 - Nucleolar protein b23 translocation after doxorubicin treatment in murine tumor cells.
11830489 - Functional role of alternatively spliced deoxycytidine kinase in sensitivity to cytarab...
10427129 - Mouse leukemia l1210 cells selected for resistance to the ribonucleotide reductase inhi...
187239 - Plasma membrane studies on drug-sensitive and -resistant cell lines. ii. ouabain sensit...
1986259 - Nucleotide pools and mutagenic effects of alkylating agents in wild-type and aprt-defic...
20214659 - Establishment and characterization of a lactating bovine mammary epithelial cell model ...
6969779 - Lyb-2 system of mouse b cells. evidence for a role in the generation of antibody-formin...
24465559 - Photodynamic therapy in pythium insidiosum - an in vitro study of the correlation of se...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-4
Journal Detail:
Title:  Journal of plant physiology     Volume:  -     ISSN:  1618-1328     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882059     Medline TA:  J Plant Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier GmbH. All rights reserved.
Affiliation:
Department of Cytophysiology, University of Łódź, ul. Pomorska 141/143, 90-236 Łódź, Poland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Development of an allele-specific, loop-mediated, isothermal amplification method (AS-LAMP) to detec...
Next Document:  Functional Interaction Analysis of GM1-Related Carbohydrates and Vibrio cholerae Toxins using Carboh...