Document Detail


Sucrose incubation increases freezing tolerance of asparagus (Asparagus officinalis L.) embryogenic cell suspensions.
MedLine Citation:
PMID:  12050778     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The freezing tolerance of asparagus (Asparagus officinalis L.) embryogenic cells, as determined by electrolyte leakage, was increased by the incubation of samples in medium containing 0.8 M sucrose. To elucidate the mechanism involved, we investigated the changes in soluble carbohydrates, cell ultrastructure and proteins accompanying the increase in freezing tolerance following incubation in sugar-rich medium. During sugar incubation, the intracellular sucrose content increased from 67 mol g-1FW to 429 mol g-1FW; it was also metabolized into fructose and glucose, as determined by high-performance liquid chromatography. Microscopy revealed that sugar incubation induced plasmolysis of embryogenic cells and drastic changes in cell ultrastructure with the appearance of rough endoplasmic reticulum (rER). Furthermore, immunoblotting analysis with anti-dehydrin antiserum revealed that a dehydrin-like protein appeared only when maximal freezing tolerance was induced by sugar incubation. These results suggest that freezing tolerance of asparagus embryogenic cells is increased by a complex mechanism involving notably changes in cell ultrastructure and accumulation of certain sugars and proteins during sugar incubation.
Authors:
Y Jitsuyama; T Suzuki; T Harada; S Fujikawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cryo letters     Volume:  23     ISSN:  0143-2044     ISO Abbreviation:  Cryo Letters     Publication Date:    2002 Mar-Apr
Date Detail:
Created Date:  2002-06-06     Completed Date:  2002-07-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9891832     Medline TA:  Cryo Letters     Country:  England    
Other Details:
Languages:  eng     Pagination:  103-12     Citation Subset:  IM    
Affiliation:
Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. y-jitsu@res.agr.hokudai.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Asparagus Plant*
Cryopreservation*
Culture Media
Electrolytes / metabolism
Freezing
Immunoblotting
Microscopy, Confocal
Microscopy, Electron
Plant Proteins / metabolism
Seeds / cytology*,  metabolism,  ultrastructure
Sucrose / metabolism,  pharmacology*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Electrolytes; 0/Plant Proteins; 57-50-1/Sucrose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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