Document Detail

Succinic acid production by Actinobacillus succinogenes using spent brewer's yeast hydrolysate as a nitrogen source.
MedLine Citation:
PMID:  19418259     Owner:  NLM     Status:  MEDLINE    
To develop a cost-effective fermentation medium, spent brewer's yeast hydrolysate was evaluated as a nitrogen source for succinic acid production by Actinobacillus succinogenes NJ113 in glucose-containing media. Autolysis and enzymatic hydrolysis were used to hydrolyze the spent brewer's yeast cells to release the nutrients. The results showed that enzymatic hydrolysis was a more effective method due to the higher succinic acid yield and cell growth. However, the incomplete glucose consumption indicated existence of nutrient limitation. Vitamins were subsequently identified as the main limiting factors for succinic acid production using enzymatically hydrolyzed spent brewer's yeast as a nitrogen source. After the addition of vitamins, cell growth and succinic acid concentration both improved. As a result, 15 g/L yeast extract could be successfully replaced with the enzymatic hydrolysate of spent brewer's yeast with vitamins supplementation, resulting in a production of 46.8 g/L succinic acid from 68 g/L glucose.
Min Jiang; Kequan Chen; Zhongmin Liu; Ping Wei; Hanjie Ying; Honam Chang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-06
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  160     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-29     Completed Date:  2010-03-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  244-54     Citation Subset:  IM    
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing, People's Republic of China.
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MeSH Terms
Actinobacillus / metabolism*
Industrial Waste*
Nitrogen / metabolism*
Saccharomyces cerevisiae / metabolism*
Succinic Acid / metabolism*
Reg. No./Substance:
0/Industrial Waste; 110-15-6/Succinic Acid; 7727-37-9/Nitrogen

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