| Successive bouts of cycling stimulates genes associated with mitochondrial biogenesis. | |
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MedLine Citation:
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PMID: 19657668 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Exercise increases mRNA for genes involved in mitochondrial biogenesis and oxidative enzyme capacity. However, little is known about how these genes respond to consecutive bouts of prolonged exercise. We examined the effects of 3 h of intensive cycling performed on three consecutive days on the mRNA associated with mitochondrial biogenesis in trained human subjects. Forty trained cyclists were tested for VO(2max) (54.7 +/- 1.1 ml kg(-1) min(-1)). The subjects cycled at 57% watts(max) for 3 h using their own bicycles on CompuTrainer Pro Model trainers (RacerMate, Seattle, WA) on three consecutive days. Muscle biopsies were obtained from the vastus lateralis pre- and post-exercise on days one and three. Muscle samples were analyzed for mRNA content of peroxisome proliferator receptor gamma coactivator-1 alpha (PGC-1alpha), sirtuin 1 (Sirt-1), cytochrome c, and citrate synthase. Data were analyzed using a 2 (time) x 2 (day) repeated measures ANOVA. Of the mRNA analyzed, the following increased from pre to post 3 h rides: cytochrome c (P = 0.006), citrate synthase (P = 0.03), PGC-1alpha (P < 0.001), and Sirt-1 (P = 0.005). The following mRNA showed significant effects from days one to three: cytochrome c (P < 0.001) and citrate synthase (P = 0.01). These data show that exhaustive cycling performed on three consecutive days resulted in both acute and chronic stimuli for mRNA associated with mitochondrial biogenesis in already trained subjects. This is the first study to illustrate an increase in sirtuin-1 mRNA with acute and chronic exercise. These data contribute to the understanding of mRNA expression during both acute and successive bouts of prolonged exercise. |
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Authors:
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Charles L Dumke; J Mark Davis; E Angela Murphy; David C Nieman; Martin D Carmichael; John C Quindry; N Travis Triplett; Alan C Utter; Sarah J Gross Gowin; Dru A Henson; Steven R McAnulty; Lisa S McAnulty |
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Publication Detail:
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Type: Clinical Trial; Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-08-06 |
Journal Detail:
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Title: European journal of applied physiology Volume: 107 ISSN: 1439-6327 ISO Abbreviation: Eur. J. Appl. Physiol. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-10-16 Completed Date: 2010-01-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100954790 Medline TA: Eur J Appl Physiol Country: Germany |
Other Details:
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Languages: eng Pagination: 419-27 Citation Subset: IM |
Affiliation:
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Department of Health and Human Performance, University of Montana, Missoula, MT 59812, USA. charles.dumke@mso.umt.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Bicycling / physiology* Citrate (si)-Synthase / genetics Cytochromes c / genetics Exercise / physiology Genes, Mitochondrial* Humans Male Mitochondria, Muscle / genetics*, metabolism, physiology* Mitochondrial Proteins / genetics Periodicity* Physical Endurance / genetics, physiology RNA, Messenger / metabolism Sirtuin 1 / genetics Up-Regulation / genetics |
| Chemical | |
Reg. No./Substance:
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0/Mitochondrial Proteins; 0/RNA, Messenger; 9007-43-6/Cytochromes c; EC 2.3.3.1/Citrate (si)-Synthase; EC 3.5.1.-/Sirtuin 1 |
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