Document Detail


Successful vitrification of mouse ovaries using less-concentrated cryoprotectants with Supercool X-1000 supplementation.
MedLine Citation:
PMID:  22234481     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The purpose of our study was to investigate the feasibility of using less-concentrated cryoprotectants supplemented with ice blocker Supercool X-1000 to vitrify ovarian tissues. Mouse ovaries were cryopreserved in different concentrations of vitrification solution alone or with Supercool X-1000, and fresh non-frozen ovaries were used as control. The proportions of morphological normality of follicles, normal GCs in follicular fluids and developing to blastocysts were higher in 12.5% ethylene glycol (EG) + 12.5% dimethylsulfoxide (DMSO) with Supercool X-1000 than those of treated in 10% EG + 10% DMSO or 15% EG + 15% DMSO alone or with Supercool X-1000. In conclusion, the inclusion of Supercool X-1000 in less-concentrated vitrification solution was effective to improve the efficiency and efficacy of cryopreservation of ovarian tissues.
Authors:
Xiuwen Tan; Enliang Song; Xiaomu Liu; Guifen Liu; Haijian Cheng; Fachun Wan
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-11
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  -     ISSN:  1543-706X     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Shandong Key Lab of Animal Disease Control and Breeding, Institute of Animal Husbandry and Veterinary Medicine, Shandong Academy of Agriculture Sciences, Jinan, Shandong, 250100, China.
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