| Successful production of intrathyroidal human T cell hybridomas: evidence for intact helper T cell function in Graves' disease. | |
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MedLine Citation:
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PMID: 2531154 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have developed a series of human intrathyroidal T-T cell hybridomas and evaluated their phenotypic characteristics and lymphokine secretions in order to further understand the role of the T cell in Graves' disease. Mitogen-stimulated T cell blasts were generated from intrathyroidal lymphocyte preparations and fused with a hypoxanthine-, aminopterin-, and thymidine-sensitive variant of the Molt 4 human leukemia T cell line. The resulting intrathyroidal T-T cell hybridomas and T-T cell hybridomas obtained from normal peripheral blood mitogen-stimulated T cell blasts were expanded and tested for their biological function. None of the generated T cell hybridomas exhibited antigen-specific IL-2 secretion when stimulated with autologous thyrocytes, although 60% of the hybridomas expressed CD3 antigen and the T cell receptor alpha/beta heterodimer. However, 9 intrathyroidal and 11 peripheral blood T cell hybridomas secreted a factor(s) that significantly enhanced immunoglobulin G secretion in vitro (P less than 0.005, by Student-Newman-Keuls test; mean +/- SEM, 338 +/- 60% increase). In summary, we have successfully used a technique that allows the construction of T-T cell hybridomas derived from intrathyroidal T cell cultures. The data demonstrated that a predominance of helper factor-secreting T cells were available for fusion within the Graves' thyroid gland. Such observations are further evidence for intact T cell help within the thyroid gland of patients with Graves' disease. |
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Authors:
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A Martin; A E Schwartz; E W Friedman; T F Davies |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of clinical endocrinology and metabolism Volume: 69 ISSN: 0021-972X ISO Abbreviation: J. Clin. Endocrinol. Metab. Publication Date: 1989 Dec |
Date Detail:
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Created Date: 1989-12-29 Completed Date: 1989-12-29 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0375362 Medline TA: J Clin Endocrinol Metab Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1104-8 Citation Subset: AIM; IM |
Affiliation:
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Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenoma
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immunology Cell Line Cells, Cultured Cytotoxicity, Immunologic Graves Disease / immunology* Humans Hybridomas / immunology* Immunoglobulin G / analysis Interleukin-2 / biosynthesis Reference Values T-Lymphocytes / immunology* T-Lymphocytes, Helper-Inducer / immunology* Thyroid Gland / immunology* Thyroid Neoplasms / immunology |
| Grant Support | |
ID/Acronym/Agency:
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DK-28242/DK/NIDDK NIH HHS; DK-35764/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin G; 0/Interleukin-2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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