Document Detail

Successful production of intrathyroidal human T cell hybridomas: evidence for intact helper T cell function in Graves' disease.
MedLine Citation:
PMID:  2531154     Owner:  NLM     Status:  MEDLINE    
We have developed a series of human intrathyroidal T-T cell hybridomas and evaluated their phenotypic characteristics and lymphokine secretions in order to further understand the role of the T cell in Graves' disease. Mitogen-stimulated T cell blasts were generated from intrathyroidal lymphocyte preparations and fused with a hypoxanthine-, aminopterin-, and thymidine-sensitive variant of the Molt 4 human leukemia T cell line. The resulting intrathyroidal T-T cell hybridomas and T-T cell hybridomas obtained from normal peripheral blood mitogen-stimulated T cell blasts were expanded and tested for their biological function. None of the generated T cell hybridomas exhibited antigen-specific IL-2 secretion when stimulated with autologous thyrocytes, although 60% of the hybridomas expressed CD3 antigen and the T cell receptor alpha/beta heterodimer. However, 9 intrathyroidal and 11 peripheral blood T cell hybridomas secreted a factor(s) that significantly enhanced immunoglobulin G secretion in vitro (P less than 0.005, by Student-Newman-Keuls test; mean +/- SEM, 338 +/- 60% increase). In summary, we have successfully used a technique that allows the construction of T-T cell hybridomas derived from intrathyroidal T cell cultures. The data demonstrated that a predominance of helper factor-secreting T cells were available for fusion within the Graves' thyroid gland. Such observations are further evidence for intact T cell help within the thyroid gland of patients with Graves' disease.
A Martin; A E Schwartz; E W Friedman; T F Davies
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of clinical endocrinology and metabolism     Volume:  69     ISSN:  0021-972X     ISO Abbreviation:  J. Clin. Endocrinol. Metab.     Publication Date:  1989 Dec 
Date Detail:
Created Date:  1989-12-29     Completed Date:  1989-12-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375362     Medline TA:  J Clin Endocrinol Metab     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1104-8     Citation Subset:  AIM; IM    
Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029.
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MeSH Terms
Adenoma / immunology
Cell Line
Cells, Cultured
Cytotoxicity, Immunologic
Graves Disease / immunology*
Hybridomas / immunology*
Immunoglobulin G / analysis
Interleukin-2 / biosynthesis
Reference Values
T-Lymphocytes / immunology*
T-Lymphocytes, Helper-Inducer / immunology*
Thyroid Gland / immunology*
Thyroid Neoplasms / immunology
Grant Support
Reg. No./Substance:
0/Immunoglobulin G; 0/Interleukin-2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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