Document Detail


Successful ongoing pregnancies after vitrification of oocytes.
MedLine Citation:
PMID:  16412739     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To demonstrate the efficiency of vitrifying mature human oocytes for different clinical indications. DESIGN: Descriptive case series. SETTING: Cryobiology laboratory, Centro Colombiano de Fertilidad y Esterilidad-CECOLFES LTDA. (Bogotá, Colombia). PATIENT(S): Oocyte vitrification was offered as an alternative management for patients undergoing infertility treatment because of ovarian hyperstimulation syndrome, premature ovarian failure, natural ovarian failure, male factor, poor response, or oocyte donation. Mature oocytes were obtained from 33 donor women and 40 patients undergoing infertility treatment. INTERVENTION(S): Oocytes were retrieved by ultrasound-guided transvaginal aspiration and vitrified with the Cryotops method, with 30% ethylene glycol, 30% dimethyl sulfoxide, and 0.5 mol/L sucrose. Viability was assessed 3 hours after thawing. The surviving oocytes were inseminated by intracytoplasmic sperm injection. Fertilization was evaluated after 24 hours. The zygotes were further cultured in vitro for up to 72 hours until time of embryo transfer. MAIN OUTCOME MEASURE(S): Recovery, viability, fertilization, and pregnancy rates. RESULT(S): Oocyte vitrification with the Cryotop method resulted in high rates of recovery, viability, fertilization, cleavage, and ongoing pregnancy. CONCLUSION(S): Vitrification with the Cryotop method is an efficient, fast, and economical method for oocyte cryopreservation that offers high rates of survival, fertilization, embryo development, and ongoing normal pregnancies, providing a new alternative for the management of female infertility.
Authors:
Elkin Lucena; Diana Patricia Bernal; Carolina Lucena; Alejandro Rojas; Abby Moran; Andrés Lucena
Related Documents :
10601099 - Birth following vitrification of a small number of human oocytes: case report.
11679519 - Vitrification of mouse oocytes using closed pulled straws (cps) achieves a high surviva...
8941049 - Endometrial preparation: lessons from oocyte donation.
17169199 - Successful pregnancy after icsi with strontium oocyte activation in low rates of fertil...
17061969 - Biomarkers of ovulation, endometrial receptivity, fertilisation, implantation and early...
1753169 - A case of human pregnancy after microinjection of capacitated sperm into the perivitell...
17449509 - The length of the fertile window is associated with the chance of spontaneously conceiv...
10796089 - Anti-d administration after childbirth for preventing rhesus alloimmunisation.
15117289 - Psychosocial predictors of successful delivery after unexplained recurrent spontaneous ...
Publication Detail:
Type:  Clinical Trial; Journal Article    
Journal Detail:
Title:  Fertility and sterility     Volume:  85     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-01-17     Completed Date:  2006-02-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  108-11     Citation Subset:  IM    
Affiliation:
Centro Colombiano de Fertilid y Esterilidad-CECOLFES LTDA., Bogotá, Colombia. cecolfes@colomsat.net.co
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Cell Survival
Cryopreservation / methods*
Female
Humans
Oocytes / cytology*
Ovarian Failure, Premature / therapy*
Ovarian Hyperstimulation Syndrome / therapy*
Pregnancy
Pregnancy Rate*
Sperm Injections, Intracytoplasmic*
Tissue Banks

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Proteomic analysis of individual human embryos to identify novel biomarkers of development and viabi...
Next Document:  Incidence and prediction of ovarian hyperstimulation syndrome in women undergoing gonadotropin-relea...