Document Detail

Successful live birth from vitrified oocytes after 5 years of cryopreservation.
MedLine Citation:
PMID:  20886366     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To report a birth of a healthy boy after long-term cryopreservation of oocytes by vitrification.
DESIGN: Clinical application.
PATIENT: A 17 year-old female with secondary pulmonary hypertension caused by transposition of great vessels visited our center in 2002, and she wished oocytes cryopreservation to avoid possible sterility after the following category X medication treatment.
INTERVENTION(S): Vitrified oocytes on Electron Microscope (EM) grids were warmed after 5 years of storage. Surviving MII oocytes were microinjected for fertilization and two embryos were transferred into a gestational carrier day 5 after microinjection.
MAIN OUTCOME MEASURE(S): Survival, fertilization, cleavage, clinical pregnancy and delivery.
RESULT(S): Eleven out of fourteen oocytes (78.6%) survived warming. Eight Metaphase II (MII) oocytes and 3 in vitro matured oocytes were microinjected; all 11 oocytes (100%) fertilized and 2 embryos were transferred on day 5. A healthy baby boy weighing 3,600 g was delivered at 38 weeks of gestation. Live-birth rates per warmed oocyte and per injected oocyte were 7.1% and 9.1% respectively.
CONCLUSION(S): Cryopreservation after vitrification with EM grids maintained the developmental competence of oocytes after long-term storage and resulted in a successful live birth.
Thomas J Kim; Seung Wook Hong
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Publication Detail:
Type:  Case Reports; Journal Article     Date:  2010-10-01
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  28     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-03-01     Completed Date:  2011-06-13     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  73-6     Citation Subset:  IM    
Reproductive Medicine Associates of New Jersey, Morristown, NJ, 07960, USA.
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MeSH Terms
Embryo Transfer
Embryonic Development
Fertilization in Vitro
Live Birth*
Oocytes / growth & development*
Time Factors
Treatment Outcome
Young Adult

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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