| Successful fertilization, pregnancy, and birth using epididymal sperm frozen 24 hours after conventional oocyte insemination. | |
| | |
MedLine Citation:
|
PMID: 7672163 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: To assess if epididymal sperm cryopreserved 24 hours after exposure to oocytes in conventional IVF can be successfully used for intracytoplasmic sperm injection (ICSI) in a subsequent cycle. DESIGN: Case report. SETTING: University of California, Irvine, Center for Reproductive Health. PATIENTS: Two men with obstructive azoospermia requiring microsurgical epididymal sperm aspiration, IVF, and ICSI. INTERVENTIONS: Freezing of epididymal sperm 24 hours after egg exposure in conventional IVF and subsequent use for assisted fertilization in a new cycle. MAIN OUTCOME MEASURE: Frozen-thawed epididymal sperm survivability and maintenance of fertilization and pregnancy capacity. RESULTS: At the time of sperm aspiration procedure (cycle I) a total of 30 oocytes were available for insemination. Of these, 15 were used for conventional IVF resulting in 2 embryos (13%) and 15 were used for ICSI, resulting in 3 embryos (20%). Sperm was cryopreserved 24 hours after conventional IVF and thawed 6 months later in a new cycle. Upon thawing, sperm were still found to be motile and at this time (cycle II) only assisted fertilization was used. Of 27 oocytes injected, 12 (44%) produced normal, cleaving embryos. One singleton pregnancy with the birth of a healthy infant girl was achieved after the tubal transfer of 5 embryos. CONCLUSION: The birth of a normal, healthy infant girl with epididymal sperm frozen 24 hours after exposure to oocytes in conventional IVF emphasizes the value of freezing any aliquot of epididymal sperm, even if the motility is very low, to avoid additional surgery in the male. From a basic science standpoint, this observation may renew interest in the study of sperm cryopreservation after occurrence of acrosome reaction and hyperactivation. |
| | |
Authors:
|
P Patrizio; T Ord; J P Balmaceda; R H Asch |
Related Documents
:
|
9241063 - Rhesus monkeys produced by nuclear transfer. 1408653 - Fever responses in newborn lambs. 21150443 - Future applications of antioxidants in premature infants. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Fertility and sterility Volume: 64 ISSN: 0015-0282 ISO Abbreviation: Fertil. Steril. Publication Date: 1995 Oct |
Date Detail:
|
Created Date: 1995-10-19 Completed Date: 1995-10-19 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 863-5 Citation Subset: IM |
Affiliation:
|
Department of Obstetrics and Gynecology, University of California, Irvine Medical Center, Orange, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cryopreservation* Epididymis Female Fertilization* Humans Infertility, Male / etiology, therapy Insemination, Artificial* / methods Labor, Obstetric* Male Micromanipulation / methods Oligospermia / complications Oocytes Pregnancy* Spermatozoa / physiology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A case of severe ovarian hyperstimulation in a healthy oocyte donor.
Next Document: Reversible alopecia after single-dose methotrexate treatment in a patient with ectopic pregnancy.