Document Detail


Successful delivery derived from vitrified-warmed spermatozoa from a patient with nonobstructive azoospermia.
MedLine Citation:
PMID:  22902062     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To report the clinical outcomes following intracytoplasmic sperm injection (ICSI) with vitrified sperm from patients with severe male factor infertility.
DESIGN: Retrospective case series.
SETTING: IVF unit of a medical center.
PATIENT(S): Three patients with severe oligozoospermia or nonobstructive azoospermia (NOA).
INTERVENTION(S): Cryopreservation of limited numbers of spermatozoa with the use of Cryotop and Cell Sleeper as nonbiologic containers.
MAIN OUTCOME MEASURE(S): Four cycles underwent intracytoplasmic sperm injection (ICSI) with vitrified sperm.
RESULT(S): A total of 148 spermatozoa in 18 containers (8.2 sperm per container) were vitrified and 36 of them (5 containers) were warmed. Thirty-three sperm (92%) were retrieved successfully and injected individually into 17 mature oocytes. Fertilization was observed in 12 oocytes (71%), and all zygotes (100%) cleaved. A couple with NOA achieved a singleton pregnancy and concluded with full-term delivery of a healthy boy (2,632 g).
CONCLUSION(S): A successful delivery was achieved after transfer of a blastocyst derived from vitrified-warmed spermatozoa. A small number of vitrified sperm cells were used for ICSI to fertilize oocytes with predictable timing.
Authors:
Yuji Endo; Yoshitaka Fujii; Shozo Kurotsuchi; Hiroaki Motoyama; Hiroaki Funahashi
Publication Detail:
Type:  Case Reports; Journal Article     Date:  2012-08-15
Journal Detail:
Title:  Fertility and sterility     Volume:  98     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-03     Completed Date:  2013-02-05     Revised Date:  2013-10-02    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1423-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Affiliation:
IVF Center, Kurashiki Medical Clinic, Kurashiki, Japan. ivf@fkmc.or.jp
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MeSH Terms
Descriptor/Qualifier:
Adult
Azoospermia / therapy*
Cryopreservation / methods*
Female
Humans
Live Birth*
Male
Pregnancy
Semen Preservation / methods*
Sperm Injections, Intracytoplasmic / methods*
Treatment Outcome
Comments/Corrections
Comment In:
J Urol. 2013 Sep;190(3):989-90   [PMID:  23931223 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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