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Subtyping of gliomas of various WHO grades by the application of immunohistochemistry.
MedLine Citation:
PMID:  24410805     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
AIMS: In 2010, four subtypes (classical, proneural, mesenchymal, and neural) of glioblastoma multiforme (GBM) were defined by molecular genetic analyses. The objective of this study was to assess whether gliomas, independently of the type and grade, could be subdivided into protein-based subtypes.
METHODS AND RESULTS: A tissue microarray (TMA) approach was applied to incorporate tissue samples of low-grade and high-grade gliomas into five TMAs. High expression levels of epidermal growth factor receptor (EGFR), CD44, c-MER proto-oncogene tyrosine kinase (MERTK), platelet-derived growth factor receptor α, p53, oligodendrocyte transcription factor 2 (OLIG2) and isocitrate dehydrogenase 1 with the R132H mutation were assessed using immunohistochemistry (IHC). Glioma could be subdivided into four subtypes by IHC. The majority of the low-grade gliomas were of the proneural subtype, i.e. high p53 expression (63% of grade II). The classical subtype, with high EGFR and low p53 expression, was most common in GBMs (39%), followed by the proneural (29%) and mesenchymal (with high CD44 and MERTK expression) (29%) subtypes, a frequency that is in line with previously published data based on molecular genetics.
CONCLUSIONS: Assessment of the expression of the five proteins EGFR, CD44, MERTK, p53 and OLIG2 is sufficient for subtyping gliomas, and can be recommended for implementation in clinical practice for both low-grade and high-grade gliomas.
Authors:
Svetlana N Popova; Michael Bergqvist; Anna Dimberg; Per-Henrik Edqvist; Simon Ekman; Göran Hesselager; Fredrik Ponten; Anja Smits; Linda Sooman; Irina Alafuzoff
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Publication Detail:
Type:  Journal Article     Date:  2013-11-05
Journal Detail:
Title:  Histopathology     Volume:  64     ISSN:  1365-2559     ISO Abbreviation:  Histopathology     Publication Date:  2014 Feb 
Date Detail:
Created Date:  2014-01-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7704136     Medline TA:  Histopathology     Country:  England    
Other Details:
Languages:  eng     Pagination:  365-79     Citation Subset:  IM    
Copyright Information:
© 2013 John Wiley & Sons Ltd.
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