Document Detail

Subtypes of bovine adenovirus type 2 exhibit major differences in region E3.
MedLine Citation:
PMID:  3739229     Owner:  NLM     Status:  MEDLINE    
The genomes of two adenovirus type 2 strains which were isolated from different hosts have been investigated. One of these strains designated ORT-111 was originally isolated from a lamb in Hungary during an outbreak of pneumoenteritis. This isolate was typed as bovine adenovirus type 2 (Ad bos 2) in a neutralization assay. The genome of ORT-111 was compared to that of the prototype strain of Ad bos 2, a virus which exclusively has been isolated from cattle. Electron microscopic heteroduplex analysis showed that 95% of the genomes were well matched, forming stable duplexes at Tm -6 degrees. Two distinct substitution loops were, however, seen which were approximately 0.5 and 1.0 kbp long. The centers of the two loops were located 5.3 and 7.7 kbp from one end of the Ad bos 2 genome. In order to map these regions relative to the gene map of human adenovirus type 2 (Ad2), restriction enzyme cleavage fragments of the two bovine viruses were cloned and hybridized to different sets of restriction fragments of human Ad2. From these results it was apparent that the centers of the two substitution loops were located at coordinates 76 and 83, respectively; thus at positions which fall within region E3 and the adjacent gene for polypeptide VIII of human Ad2. The observed differences between the genomes of the two Ad bos 2 strains are in sharp contrast to those previously observed when the genomes of different human adenovirus serotypes were compared. In the latter case the hexon and the fiber genes showed the most pronounced variation.
S Belák; A Virtanen; J Zabielski; M Rusvai; G Berencsi; U Pettersson
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virology     Volume:  153     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  1986 Sep 
Date Detail:
Created Date:  1986-09-17     Completed Date:  1986-09-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  262-71     Citation Subset:  IM    
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MeSH Terms
Adenoviridae / genetics*,  immunology,  pathogenicity
Adenoviruses, Human / genetics
Cattle / microbiology
Chromosome Mapping
Cloning, Molecular
DNA, Viral / analysis
Genes, Viral*
Nucleic Acid Hybridization
Species Specificity
Reg. No./Substance:
0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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