Document Detail


Subtype identification of Blastocystis spp. isolated from patients in a major hospital in northeastern Thailand.
MedLine Citation:
PMID:  23224731     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The present study is aimed to identify the prevalence of Blastocystis subtypes isolated from patients in a major hospital in northeastern Thailand. A total of 562 stool samples were examined by culture technique, and 56 Blastocystis-positive samples were analyzed further by the combination of restriction fragment length polymorphism (RFLP) followed by polymerase chain reaction with sequence-tagged site primers (PCR-STS). By RFLP profiles, Blastocystis genotypes were categorized into four groups: group A (12, 21.4 %), group B (32, 57.1 %), group C (10, 17.9 %), and group D (2, 3.6 %). By PCR-STS, only four subtypes were identified. All 12 (21.4 %) isolates in group A were identified as subtype 1. Similarly, all 32 (57.1 %) isolates in group B were subtype 3. In group C, 10 (17.9 %) samples were all subtype 7, and two samples (3.6 %) in group D were both subtype 6. Of 56 Blastocystis-positive patients, 31 (55.4 %) were asymptomatic and 22 (39.4 %) have gastrointestinal symptoms. No significant association was observed between the Blastocystis subtypes and the clinical features. Among the Blastocystis-positive patients, the most characteristic stool samples were loose (78.6 %) and soft (17.9 %). In conclusion, the most common Blastocystis spp. in northeastern Thailand was subtype 3 followed by subtype 1. Relatively minor subtypes, subtype 6 and subtype 7 which are considered as avian subtypes, were found for the first time in humans in Thailand.
Authors:
Sarinee Jantermtor; Porntip Pinlaor; Kookwan Sawadpanich; Somchai Pinlaor; Arunnee Sangka; Chotechana Wilailuckana; Wachanan Wongsena; Hisao Yoshikawa
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-9
Journal Detail:
Title:  Parasitology research     Volume:  -     ISSN:  1432-1955     ISO Abbreviation:  Parasitol. Res.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8703571     Medline TA:  Parasitol Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Graduate School, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.
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