Document Detail


Subtraction-coupled custom microarray analysis for gene discovery and gene expression studies in the CNS.
MedLine Citation:
PMID:  11923191     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The revolution in our knowledge about the genomes of organisms gives rise to the question, what do we do with this information? The development of techniques allowing high throughput analysis of RNA and protein expression, such as cDNA microarrays, provide for genome-wide analysis of gene expression. These analyses will help bridge the gap between systems and molecular neuroscience. This review discusses the advantages of using a subtractive hybridization technique, such as a representational difference analysis, to generate a custom cDNA microarray enriched for genes relevant to investigating complex, heterogeneous tissues such as those involved in the chemical senses. Real and hypothetical examples of these experiments are discussed. Benefits of this approach over traditional microarray techniques include having a more relevant clone set, the potential for gene discovery and the creation of a new tool to investigate similar systems. Potential pitfalls may include PCR artifacts and the need for sequencing. However, these disadvantages can be overcome so that the coupling of subtraction techniques to microarray screening can be a fruitful approach to a variety of experimental systems.
Authors:
Joseph D Dougherty; Daniel H Geschwind
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Chemical senses     Volume:  27     ISSN:  0379-864X     ISO Abbreviation:  Chem. Senses     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-03-29     Completed Date:  2002-09-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8217190     Medline TA:  Chem Senses     Country:  England    
Other Details:
Languages:  eng     Pagination:  293-8     Citation Subset:  IM    
Affiliation:
Interdepartmental Program in Neuroscience, University of California-Los Angeles, Los Angeles, CA 90094, USA.
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MeSH Terms
Descriptor/Qualifier:
Central Nervous System / metabolism*
Gene Expression Profiling*
Oligonucleotide Array Sequence Analysis*
Subtraction Technique*
Grant Support
ID/Acronym/Agency:
MH 60233/MH/NIMH NIH HHS; NS 41393/NS/NINDS NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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