Document Detail


Substratum-dependent and region-specific control of attachment and proliferation of gastrointestinal epithelial cells in primary serum-free culture.
MedLine Citation:
PMID:  9015357     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A system for the primary serum-free culture of fetal rat gastrointestinal epithelial cells was used to examine the role of the extracellular matrix (ECM) in the attachment and proliferation of these epithelial cells. Forestomach epithelial cells (FSEC) were able to attach to and proliferate on plastic dishes without a substratum, while glandular stomach epithelial cells (GSEC) and duodenal epithelial cells (DEC) were unable to do so. The presence of a substratum promoted the attachment and proliferation of these epithelial cells. The effects of various components of the ECM differed depending on the type of cell. FSEC attached most efficiently to a substratum of fibronectin, while GSEC did so to laminin. DEC attached more efficiently to type I collagen and fibronectin than to any other substratum. FSEC proliferated most rapidly on laminin, while GSEC and DEC did so on collagen gels. These substrata induced the most efficient attachment and proliferation of FSEC, and they were effective in promoting the attachment and proliferation of GSEC and DEC in decreasing order of efficiency, indicating the existence of a head-to-tail gradient in the response of epithelial cells to substrata. The expression of c-myc mRNA in these cells differed depending upon the substratum on which they were cultured and the mRNA level was well correlated with the extent of the cell proliferation, indicating that the cell proliferation is mediated by c-myc gene expression, which is regulated by cell-ECM interactions. The results of the present study demonstrate that proliferation of gastrointestinal epithelial cells is regulated region-specifically not only by soluble factors but also by insoluble components of the ECM.
Authors:
M Ichinose; N Yahagi; Y Matsubara; S Tsukada; M Oka; Y Shimizu; S Yonezawa; T Kageyama; K Miki; H Fukamachi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  230     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-02-19     Completed Date:  1997-02-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  537-41     Citation Subset:  IM    
Affiliation:
First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Adhesion
Cell Division
Culture Media, Serum-Free
Digestive System / cytology*,  metabolism
Duodenum / cytology,  metabolism
Epithelial Cells
Epithelium / metabolism
Extracellular Matrix / physiology*
Fetus
Gene Expression Regulation
Genes, myc
Rats
Rats, Inbred F344
Stomach / cytology,  metabolism
Chemical
Reg. No./Substance:
0/Culture Media, Serum-Free

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