Document Detail

Substrates and supplements for hESCs: a critical review.
MedLine Citation:
PMID:  23288664     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Different laboratories around the world have succeeded in establishing human embryonic stem cell (hESC) lines. However, culture conditions vary considerably among the protocols used and the vast majority of the lines at some stage of their creation have been in contact with an animal derived component. One of the main problems to be overcome for the generation of a clinical-grade hESC line is the choice of a substrate and medium that allows derivation and culture, where animal derived components are kept to a minimum or completely excluded.
MATERIALS AND METHODS: The following review describes past and more recent achievements in the creation and culturing of hESC. It describes protocols, giving special attention to the matrices and supplements used for derivation, maintainance and cryostorage, considering whether they included defined, undefined and/or animal-derived components in their formulations.
CONCLUSION: This information shall be useful for the creation and choice of new substrates and supplements for future research in the field of hESC for therapeutic purposes.
Melisa Candela Crocco; Nilo Fratnz; Adriana Bos-Mikich
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2013-01-04
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  30     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-26     Completed Date:  2013-09-23     Revised Date:  2014-03-07    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  315-23     Citation Subset:  IM    
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MeSH Terms
Cell Culture Techniques / methods*
Cell Line / cytology*
Drug Combinations
Embryonic Stem Cells / cytology*
Feeder Cells / cytology
Fibroblasts / cytology
Reg. No./Substance:
0/Drug Combinations; 0/Laminin; 0/Proteoglycans; 119978-18-6/matrigel; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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