| Substrate specificity screening of oat (Avena sativa) seeds aminopeptidase demonstrate unusually broad tolerance in S1 pocket. | |
| | |
MedLine Citation:
|
PMID: 22366636 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Aminopeptidases are proteolytic enzymes that remove one amino acid at a time from N-terminus of peptidic substrates. In plants, inhibitors of aminopeptidases can find potential applications in agriculture as herbicides. In this report we have used a library of fluorogenic derivatives of natural and unnatural amino acids for substrate specificity profiling of oat (Avena sativa) aminopeptidase. Interestingly, we have found that this enzyme recognizes effectively among the natural amino acids basic residues like Arg and Lys, hydrophobic Phe, Leu and Met, but also to some extent acidic residues Asp and Glu. In the case of unnatural amino acids hydrophobic residues (hPhe and hCha) and basic hArg were preferentially recognized. |
| | |
Authors:
|
Anna D Gajda; Małgorzata Pawełczak; Marcin Drag |
Related Documents
:
|
3989556 - A study on erythrocyte membrane plasmalogen in myotonic dystrophy. 1834476 - Metabolism of linolenic acid and docosahexaenoic acid in rat retinas and rod outer segm... 1811436 - Giardia lamblia: phospholipid analysis of human isolates. 18204906 - Association of fatty acids in serum phospholipids with lung function and bronchial hype... 2998446 - The digestion of fibre by pigs. 2. volatile fatty acid concentrations in large intestin... 1992816 - Effects of pregnancy and ovarian steroids on fatty acid synthesis and uptake in syrian ... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-2-8 |
Journal Detail:
|
Title: Plant physiology and biochemistry : PPB / Societe francaise de physiologie vegetale Volume: 54C ISSN: 1873-2690 ISO Abbreviation: - Publication Date: 2012 Feb |
Date Detail:
|
Created Date: 2012-2-27 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9882449 Medline TA: Plant Physiol Biochem Country: - |
Other Details:
|
Languages: ENG Pagination: 6-9 Citation Subset: - |
Copyright Information:
|
Copyright © 2012 Elsevier Masson SAS. All rights reserved. |
Affiliation:
|
Department of Medicinal Chemistry and Microbiology, Faculty of Chemistry, Wroclaw University of Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: High resolution electron backscatter diffraction measurements of elastic strain variations in the pr...
Next Document: Temporal accumulation of salicylic acid activates the defense response against Colletotrichum in str...