Document Detail


Substrate specificity and regioselectivity of delta12 and omega3 fatty acid desaturases from Saccharomyces kluyveri.
MedLine Citation:
PMID:  19060400     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Delta12 and omega3 fatty acid desaturases are key enzymes in the synthesis of polyunsaturated fatty acids (PUFAs), which are important constituents of membrane glycerolipids and also precursors to signaling molecules in many organisms. In this study, we determined the substrate specificity and regioselectivity of the Delta12 and omega3 fatty acid desaturases from Saccharomyces kluyveri (Sk-FAD2 and Sk-FAD3). Based on heterologous expression in Saccharomyces cerevisiae, it was found that Sk-FAD2 converted C16-20 monounsaturated fatty acids to diunsaturated fatty acids by the introduction of a second double bond at the nu+3 position, while Sk-FAD3 recognized the omega3 position of C18 and C20. Furthermore, fatty acid analysis of major phospholipids suggested that Sk-FAD2 and Sk-FAD3 have no strong substrate specificity toward the lipid polar head group or the sn-positions of fatty acyl groups in phospholipids.
Authors:
Takahiro Oura; Susumu Kajiwara
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  72     ISSN:  1347-6947     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-24     Completed Date:  2009-02-11     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  3174-9     Citation Subset:  IM    
Affiliation:
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa, Japan. toura@bio.titech.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Fatty Acid Desaturases / metabolism*
Fatty Acids / chemistry,  metabolism
Gene Expression
Phospholipids / chemistry,  metabolism
Saccharomyces / enzymology*
Saccharomyces cerevisiae / genetics
Stereoisomerism
Substrate Specificity
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Phospholipids; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.14.99.-/delta-12 fatty acid desaturase; EC 1.14.99.-/omega-3 fatty acid desaturase

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