| Substrate specificity and regioselectivity of delta12 and omega3 fatty acid desaturases from Saccharomyces kluyveri. | |
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MedLine Citation:
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PMID: 19060400 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Delta12 and omega3 fatty acid desaturases are key enzymes in the synthesis of polyunsaturated fatty acids (PUFAs), which are important constituents of membrane glycerolipids and also precursors to signaling molecules in many organisms. In this study, we determined the substrate specificity and regioselectivity of the Delta12 and omega3 fatty acid desaturases from Saccharomyces kluyveri (Sk-FAD2 and Sk-FAD3). Based on heterologous expression in Saccharomyces cerevisiae, it was found that Sk-FAD2 converted C16-20 monounsaturated fatty acids to diunsaturated fatty acids by the introduction of a second double bond at the nu+3 position, while Sk-FAD3 recognized the omega3 position of C18 and C20. Furthermore, fatty acid analysis of major phospholipids suggested that Sk-FAD2 and Sk-FAD3 have no strong substrate specificity toward the lipid polar head group or the sn-positions of fatty acyl groups in phospholipids. |
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Authors:
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Takahiro Oura; Susumu Kajiwara |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-07 |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 72 ISSN: 1347-6947 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-12-24 Completed Date: 2009-02-11 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 3174-9 Citation Subset: IM |
Affiliation:
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Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa, Japan. toura@bio.titech.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Fatty Acid Desaturases
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metabolism* Fatty Acids / chemistry, metabolism Gene Expression Phospholipids / chemistry, metabolism Saccharomyces / enzymology* Saccharomyces cerevisiae / genetics Stereoisomerism Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Phospholipids; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.14.99.-/delta-12 fatty acid desaturase; EC 1.14.99.-/omega-3 fatty acid desaturase |
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