Document Detail


Substrate specificity of acyl-Delta(6)-desaturases from Continental versus Macaronesian Echium species.
MedLine Citation:
PMID:  16455116     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Echium (Boraginaceae) species from the Macaronesian islands exhibit an unusually high level of gamma-linolenic acid (18:3n-6; GLA) and relatively low content of octadecatetraenoic acid (18:4n-3; OTA) in the seed, while the amounts of both fatty acids in their Continental (European) relatives are rather similar. We have tested the hypothesis of whether a different specificity of the acyl-Delta(6)-desaturases (D6DES) towards their respective usual substrates, linoleic acid (18:2n-6; LA) for GLA and alpha-linolenic acid (18:3n-3; ALA) for OTA, was partly responsible for this composition pattern. To this aim we have expressed in yeast the coding sequences of the D6DES genes for the Continental species Echium sabulicola, and the Macaronesian Echium gentianoides. When the yeast cultures are supplemented with the two fatty acid substrates (LA and ALA), a similar utilization of both compounds was found for the D6DES of E. sabulicola, while a preference for LA over ALA was observed for the enzyme of E. gentianoides. This substrate preference must contribute to the increased accumulation of GLA in the seeds of the Macaronesian Echium species. Comparison among the amino acid sequences of these desaturases and other related enzymes, allowed us the discussion about the possible involvement of some specific positions in the determination of substrate specificity.
Authors:
Federico García-Maroto; Aurora Mañas-Fernández; José A Garrido-Cárdenas; Diego López Alonso
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-02-07
Journal Detail:
Title:  Phytochemistry     Volume:  67     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-20     Completed Date:  2006-05-03     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  540-4     Citation Subset:  IM    
Affiliation:
Area de Bioquímica, Facultad de Ciencias Experimentales, Universidad de Almería, 04120 Almería, Spain.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Echium / classification*,  enzymology*,  genetics
Fatty Acid Desaturases / chemistry,  genetics,  metabolism*
Fatty Acids / chemistry,  metabolism
Molecular Sequence Data
Plant Oils / chemistry
Seeds / chemistry
Sequence Alignment
Sequence Homology
Sequence Homology, Amino Acid
Substrate Specificity
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Plant Oils; EC 1.14.19.-/Fatty Acid Desaturases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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