Document Detail


Substrate responses to acid-base alterations in dog renal proximal tubules.
MedLine Citation:
PMID:  1621808     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Proximal tubules from dog kidney were incubated for 2-6 min with low concentrations of pyruvate, glutamine, and malate. When initial medium citrate was between 0 and 0.5 mM and alpha-ketoglutarate was between 0 and 0.1 mM, concentrations of these two substrates in tubules and media after incubation were lower with 10 than with 40 mM HCO3-. Malate levels in tubules and media changed in the opposite direction. CO2 formation from labeled citrate or alpha-ketoglutarate was greater at low than at high HCO3-. In tubules treated with digitonin to disrupt the cell membrane, differences in citrate and alpha-ketoglutarate concentrations in tubules at high and low HCO3- were eliminated. The effects of acid-base changes seen in intact tubules on malate levels and on the rate of oxidation of labeled citrate persisted after digitonin pretreatment. These results show that acute effects of acid-base changes on citrate, alpha-ketoglutarate, and malate levels observed in intact renal cortex can be reproduced in isolated tubules. They suggest that these changes are related to changes in levels of citrate and alpha-ketoglutarate in cytoplasm without corresponding changes within mitochondria.
Authors:
D P Simpson; N McSherry; E Scarbrough; K Zweifel
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  262     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1992-08-04     Completed Date:  1992-08-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  F1039-46     Citation Subset:  IM    
Affiliation:
Department of Medicine, University of Wisconsin-Madison 53792.
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MeSH Terms
Descriptor/Qualifier:
Acid-Base Equilibrium*
Animals
Antacids / pharmacology
Bicarbonates / pharmacology
Carbon Dioxide / metabolism
Citrates / pharmacology
Citric Acid
Culture Media
Digitonin / pharmacology
Dogs
Ketoglutaric Acids / pharmacology
Kidney Tubules, Proximal / drug effects,  metabolism*
Time Factors
Grant Support
ID/Acronym/Agency:
R01-DK-18351/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Antacids; 0/Bicarbonates; 0/Citrates; 0/Culture Media; 0/Ketoglutaric Acids; 11024-24-1/Digitonin; 124-38-9/Carbon Dioxide; 328-50-7/alpha-ketoglutaric acid; 77-92-9/Citric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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