Document Detail

Substrate preference of metalloproteinases secreted by ts-110 Moloney murine sarcoma virus-transformed normal rat kidney cells.
MedLine Citation:
PMID:  1449476     Owner:  NLM     Status:  MEDLINE    
In this study, the two forms of affinity-purified transformation-associated proteins (TAPs) (68 and 64 kD) were shown to have different substrate preferences. For the 68-kD TAP, the order of substrate preference was collagen types I, III, and V; fibronectin; gelatin; and collagen IV. For the 64-kD TAP, the order of substrate preference was collagen I, III, and V and gelatin. The 64-kD TAP did not cleave collagen IV and fibronectin. We also found a 71-kD metalloproteinase in the concentrated purified TAPs that reacted only weakly with a TAP monoclonal antibody and showed this substrate preference: collagen I, III, and V; gelatin; and collagen IV. Whether this 71-kD TAP is a new member of the rat metalloproteinase family will be investigated.
H Z Zhang; M Scanlon; J L Murray; J C Chan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  189     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1992 Nov 
Date Detail:
Created Date:  1992-12-30     Completed Date:  1992-12-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  21-6     Citation Subset:  IM    
Department of Tumor Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
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MeSH Terms
Antibodies, Monoclonal
Blotting, Western
Cell Line, Transformed
Cell Transformation, Neoplastic*
Collagen / metabolism
Electrophoresis, Polyacrylamide Gel
Metalloendopeptidases* / isolation & purification,  metabolism*
Molecular Weight
Moloney murine sarcoma virus / genetics*
Substrate Specificity
Grant Support
Reg. No./Substance:
0/Antibodies; 0/Antibodies, Monoclonal; 9007-34-5/Collagen; EC 3.4.24.-/Metalloendopeptidases

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