Document Detail


Substrate-dependent differences in growth and biological properties of fibroblasts and epithelial cells grown in microcarrier culture.
MedLine Citation:
PMID:  2985616     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Normal diploid human fibroblasts and first passage monkey kidney epithelial cells were examined for growth and metabolic activity on microcarriers made from glass and on microcarriers made from DEAE-dextran. The cells grew to a higher density (cells cm2 of surface area) on the glass microcarriers made from glass and on microcarriers made from DEAE-dextran. The cells grew to a higher density (cells/cm2 of surface area) on the glass microcarriers than they did on the DEAE-dextran microcarriers and morphological differences were observed between the cells growing on the two substrates. On the DEAE-dextran microcarriers, the cells were much more resistant to protease-mediated detachment than were the cells on the glass microcarriers. In these respects, the cells grown on the glass microcarriers were similar to cells grown in conventional monolayer culture. Interestingly, the cells grown on the DEAE-dextran microcarriers expressed higher levels of proteolytic enzyme activity than the cells grown on the glass microcarriers. Substrate-dependent differences in prostaglandin production also occurred--both in unstimulated cells and in cells stimulated with 12-0-tetradecanoyl phorbol acetate. The unstimulated cells on the glass microcarriers produced slightly higher levels of three different prostaglandins than did the cells on the DEAE-dextran microcarriers. However, after stimulation the levels were much higher in the DEAE-dextran microcarrier cultures than in the glass microcarrier cultures. In contrast to these results, there was no significant, substrate-dependent difference in the production of infectious herpes simplex virus. Taken together, these findings suggest that when commercially-useful cells such as normal fibroblasts and epithelial cells are grown in large quantities on microcarriers, the nature of the substrate may have a profound effect on the growth and physiology of the cells. They also suggest that when microcarriers are used, unexpected results based on preliminary work in conventional monolayer culture may be obtained.
Authors:
J Varani; M Dame; J Rediske; T F Beals; W Hillegas
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of biological standardization     Volume:  13     ISSN:  0092-1157     ISO Abbreviation:  J Biol Stand     Publication Date:  1985 Jan 
Date Detail:
Created Date:  1985-06-17     Completed Date:  1985-06-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0400335     Medline TA:  J Biol Stand     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  67-76     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Cercopithecus aethiops
Cytological Techniques / instrumentation
DEAE-Dextran
Epithelial Cells
Fibroblasts / cytology*,  metabolism,  microbiology
Humans
Microscopy, Electron, Scanning
Peptide Hydrolases / metabolism
Prostaglandin-Endoperoxide Synthases / metabolism
Prostaglandins / biosynthesis
Simplexvirus / growth & development
Substrate Specificity
Time Factors
Trypsin / pharmacology
Grant Support
ID/Acronym/Agency:
CA36656/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Prostaglandins; 9015-73-0/DEAE-Dextran; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases; EC 3.4.-/Peptide Hydrolases; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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