| Substrate-dependent differences in growth and biological properties of fibroblasts and epithelial cells grown in microcarrier culture. | |
MedLine Citation:
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PMID: 2985616 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Normal diploid human fibroblasts and first passage monkey kidney epithelial cells were examined for growth and metabolic activity on microcarriers made from glass and on microcarriers made from DEAE-dextran. The cells grew to a higher density (cells cm2 of surface area) on the glass microcarriers made from glass and on microcarriers made from DEAE-dextran. The cells grew to a higher density (cells/cm2 of surface area) on the glass microcarriers than they did on the DEAE-dextran microcarriers and morphological differences were observed between the cells growing on the two substrates. On the DEAE-dextran microcarriers, the cells were much more resistant to protease-mediated detachment than were the cells on the glass microcarriers. In these respects, the cells grown on the glass microcarriers were similar to cells grown in conventional monolayer culture. Interestingly, the cells grown on the DEAE-dextran microcarriers expressed higher levels of proteolytic enzyme activity than the cells grown on the glass microcarriers. Substrate-dependent differences in prostaglandin production also occurred--both in unstimulated cells and in cells stimulated with 12-0-tetradecanoyl phorbol acetate. The unstimulated cells on the glass microcarriers produced slightly higher levels of three different prostaglandins than did the cells on the DEAE-dextran microcarriers. However, after stimulation the levels were much higher in the DEAE-dextran microcarrier cultures than in the glass microcarrier cultures. In contrast to these results, there was no significant, substrate-dependent difference in the production of infectious herpes simplex virus. Taken together, these findings suggest that when commercially-useful cells such as normal fibroblasts and epithelial cells are grown in large quantities on microcarriers, the nature of the substrate may have a profound effect on the growth and physiology of the cells. They also suggest that when microcarriers are used, unexpected results based on preliminary work in conventional monolayer culture may be obtained. |
Authors:
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J Varani; M Dame; J Rediske; T F Beals; W Hillegas |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of biological standardization Volume: 13 ISSN: 0092-1157 ISO Abbreviation: J Biol Stand Publication Date: 1985 Jan |
Date Detail:
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Created Date: 1985-06-17 Completed Date: 1985-06-17 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0400335 Medline TA: J Biol Stand Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 67-76 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Cercopithecus aethiops Cytological Techniques / instrumentation DEAE-Dextran Epithelial Cells Fibroblasts / cytology*, metabolism, microbiology Humans Microscopy, Electron, Scanning Peptide Hydrolases / metabolism Prostaglandin-Endoperoxide Synthases / metabolism Prostaglandins / biosynthesis Simplexvirus / growth & development Substrate Specificity Time Factors Trypsin / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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CA36656/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Prostaglandins; 9015-73-0/DEAE-Dextran; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases; EC 3.4.-/Peptide Hydrolases; EC 3.4.21.4/Trypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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