Document Detail


Substantial expression of mature elastin in arterial constructs.
MedLine Citation:
PMID:  21282618     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mature elastin synthesis is a key challenge in arterial tissue engineering. Most engineered vessels lack elastic fibers in the medial layer and those present are poorly organized. The objective of this study is to increase mature elastin synthesis in small-diameter arterial constructs. Adult primary baboon smooth muscle cells (SMCs) were seeded in the lumen of porous tubular scaffolds fabricated from a biodegradable elastomer, poly(glycerol sebacate) (PGS) and cultured in a pulsatile flow bioreactor for 3 wk. We tested the effect of pore sizes on construct properties by histological, biochemical, and mechanical evaluations. Histological analysis revealed circumferentially organized extracellular matrix proteins including elastin and the presence of multilayered SMCs expressing calponin and α-smooth muscle actin. Biochemical analysis demonstrated that the constructs contained mature elastin equivalent to 19% of the native arteries. Mechanical tests indicated that the constructs could withstand up to 200 mmHg burst pressure and exhibited compliance comparable to native arteries. These results show that nontransfected cells in PGS scaffolds in unsupplemented medium produced a substantial amount of mature elastin within 3 wk and the elastic fibers had similar orientation as those in native arteries. The 25-32 μm pore size supported cell organization and elastin synthesis more than larger pore sizes. To our knowledge, there was no prior report of the synthesis of mature and organized elastin in arterial constructs without exogenous factors or viral transduction.
Authors:
Kee-Won Lee; Donna B Stolz; Yadong Wang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-01-31
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  108     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-17     Completed Date:  2011-04-14     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2705-10     Citation Subset:  IM    
Affiliation:
Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15261, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arteries / anatomy & histology*,  metabolism
Bioreactors*
Decanoates
Elastin / biosynthesis*,  metabolism
Fluorescent Antibody Technique
Glycerol / analogs & derivatives
Materials Testing
Microscopy, Electron, Scanning
Myocytes, Smooth Muscle / metabolism*
Papio
Polymers
Regenerative Medicine / methods
Tissue Engineering / methods*
X-Ray Microtomography
Grant Support
ID/Acronym/Agency:
R01 HL089658/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Decanoates; 0/Polymers; 0/poly(glycerol-sebacate); 56-81-5/Glycerol; 9007-58-3/Elastin
Comments/Corrections

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