| Substantial CCT activity is required for cell cycle progression and cytoskeletal organization in mammalian cells. | |
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MedLine Citation:
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PMID: 16765944 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The chaperonin CCT hexadecamer is required for the folding of non-native actins and tubulins in eukaryotic cells. Among the consequences of greatly reducing CCT holocomplex levels in human cell lines by siRNA targeting are growth arrest and changes in cell morphology and motility. Less extensive reduction of CCT activity via microinjection of an inhibitory anti-CCT epsilon subunit monoclonal antibody, which alters the rates of substrate processing by CCT in vitro, causes a delay in cell cycle progression through G1/S phase in synchronized Swiss 3T3 cells. The degree of growth arrest strongly correlates with the extent of CCT depletion, indicating that full CCT activity is required for normal cell growth and division. Depletion of CCT does not affect actin polypeptide synthesis but causes a reduction in levels of native actin and perturbation of actin-based cell motility in BE cells. There are no large-scale effects on cytoplasmic protein synthesis or a general heat shock response during periods of low CCT activity. |
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Authors:
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Julie Grantham; Karen I Brackley; Keith R Willison |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental cell research Volume: 312 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-07-20 Completed Date: 2006-08-31 Revised Date: 2011-11-02 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: United States |
Other Details:
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Languages: eng Pagination: 2309-24 Citation Subset: IM |
Affiliation:
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Cancer Research UK Centre for Cell and Molecular Biology, Chester Beatty Laboratories, The Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK. julie.grantham@molbio.gu.se |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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biosynthesis,
metabolism Animals Antibodies, Monoclonal / pharmacology Apoptosis / physiology Cell Cycle / drug effects, physiology* Cell Cycle Proteins / metabolism Cell Line, Tumor Cell Movement / physiology Cell Proliferation Chaperonin Containing TCP-1 Chaperonins / genetics, immunology, metabolism* Cyclin E / biosynthesis Cytoskeleton / drug effects, metabolism* HSP90 Heat-Shock Proteins / metabolism Hela Cells Humans Mice Microfilaments / metabolism Microtubules / metabolism Protein Biosynthesis / genetics Protein-Serine-Threonine Kinases / metabolism Proto-Oncogene Proteins / metabolism RNA, Small Interfering / genetics Swiss 3T3 Cells Tubulin / biosynthesis, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Antibodies, Monoclonal; 0/CCT5 protein, human; 0/Cell Cycle Proteins; 0/Cyclin E; 0/HSP90 Heat-Shock Proteins; 0/Proto-Oncogene Proteins; 0/RNA, Small Interfering; 0/Tcp1 protein, mouse; 0/Tubulin; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/polo-like kinase 1; EC 3.6.1.-/Chaperonin Containing TCP-1; EC 3.6.1.-/Chaperonins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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