Document Detail

Substance P receptor-mediated secretion of respiratory glycoconjugate from feline airways in vitro.
MedLine Citation:
PMID:  2465145     Owner:  NLM     Status:  MEDLINE    
The effect of substance P (SP) and other tachykinins on respiratory glycoconjugate (RGC) release was studied in a feline tracheal organ culture system. SP in concentrations of 10(-5) and 10(-6) M stimulated an increase in RGC release of 35 +/- 8% and 18.5 +/- 5%, respectively. The addition of the protease inhibitor aprotinin or the enkephalinase inhibitor thiorphan to the cultures had no effect on the baseline secretion of RGC but markedly potentiated the activity of SP. SP in the presence of aprotinin or thiorphan was active at 10(-8) -10(-9) M concentrations and was more potent at each concentration studied (in the presence of peptidase inhibitors). Among other tachykinins studied, only physalaemin in the presence of aprotinin had a clear stimulatory effect on RGC release at 10(-6) M concentration (26% +/- 5% increase above control, n = 4, p less than 0.02); kassinin, neurokinin A, and neurokinin B had little or no effect on RGC secretion in concentrations of 10(-6) M or less. Autoradiographic studies of [125I]SP binding revealed SP receptor expression in the submucosal glands of the feline trachea. [125I]SP binding was inhibited in the presence of excess unlabeled SP. We conclude that SP receptors are present in the feline tracheal submucosal glands and that binding to SP receptors results in RGC secretion.
J D Lundgren; C J Wiedermann; C Logun; J Plutchok; M Kaliner; J H Shelhamer
Related Documents :
7509285 - Effect of scyliorhinin i and synthetic scyliorhinin i derivatives at mammalian tachykin...
18556225 - Nk(3) receptor agonism promotes episodic-like memory in mice.
7865755 - Nk1 and nk2 receptors contribute to c-fibre evoked slow potentials in the spinal cord.
11447015 - Tachykinins mediate slow excitatory postsynaptic transmission in guinea pig sphincter o...
24302675 - The bitter taste receptor t2r38 is an independent risk factor for chronic rhinosinusiti...
16166225 - Effect of cholesterol depletion on mitogenesis and survival: the role of caveolar and n...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental lung research     Volume:  15     ISSN:  0190-2148     ISO Abbreviation:  Exp. Lung Res.     Publication Date:  1989  
Date Detail:
Created Date:  1989-04-04     Completed Date:  1989-04-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8004944     Medline TA:  Exp Lung Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  17-29     Citation Subset:  IM    
Critical Care Medicine Department, Clinical Center, NIH, Bethesda, Maryland 20892.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Culture Techniques
Glycoconjugates / secretion*
Mucous Membrane / metabolism
Osmolar Concentration
Receptors, Neurokinin-1
Receptors, Neurotransmitter / metabolism,  physiology*
Substance P / pharmacology
Tachykinins / pharmacology
Time Factors
Trachea / metabolism,  secretion*
Reg. No./Substance:
0/Glycoconjugates; 0/Receptors, Neurokinin-1; 0/Receptors, Neurotransmitter; 0/Tachykinins; 33507-63-0/Substance P

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Effect of murine gamma interferon on the cellular responses to bleomycin in mice.
Next Document:  Photomorphogenesis in Physarum polycephalum. Temporal expression pattern of actin, alpha- and beta-t...