Document Detail


Substance P receptor-mediated secretion of respiratory glycoconjugate from feline airways in vitro.
MedLine Citation:
PMID:  2465145     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of substance P (SP) and other tachykinins on respiratory glycoconjugate (RGC) release was studied in a feline tracheal organ culture system. SP in concentrations of 10(-5) and 10(-6) M stimulated an increase in RGC release of 35 +/- 8% and 18.5 +/- 5%, respectively. The addition of the protease inhibitor aprotinin or the enkephalinase inhibitor thiorphan to the cultures had no effect on the baseline secretion of RGC but markedly potentiated the activity of SP. SP in the presence of aprotinin or thiorphan was active at 10(-8) -10(-9) M concentrations and was more potent at each concentration studied (in the presence of peptidase inhibitors). Among other tachykinins studied, only physalaemin in the presence of aprotinin had a clear stimulatory effect on RGC release at 10(-6) M concentration (26% +/- 5% increase above control, n = 4, p less than 0.02); kassinin, neurokinin A, and neurokinin B had little or no effect on RGC secretion in concentrations of 10(-6) M or less. Autoradiographic studies of [125I]SP binding revealed SP receptor expression in the submucosal glands of the feline trachea. [125I]SP binding was inhibited in the presence of excess unlabeled SP. We conclude that SP receptors are present in the feline tracheal submucosal glands and that binding to SP receptors results in RGC secretion.
Authors:
J D Lundgren; C J Wiedermann; C Logun; J Plutchok; M Kaliner; J H Shelhamer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental lung research     Volume:  15     ISSN:  0190-2148     ISO Abbreviation:  Exp. Lung Res.     Publication Date:  1989  
Date Detail:
Created Date:  1989-04-04     Completed Date:  1989-04-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8004944     Medline TA:  Exp Lung Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  17-29     Citation Subset:  IM    
Affiliation:
Critical Care Medicine Department, Clinical Center, NIH, Bethesda, Maryland 20892.
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoradiography
Cats
Culture Techniques
Glycoconjugates / secretion*
Mucous Membrane / metabolism
Osmolar Concentration
Receptors, Neurokinin-1
Receptors, Neurotransmitter / metabolism,  physiology*
Substance P / pharmacology
Tachykinins / pharmacology
Time Factors
Trachea / metabolism,  secretion*
Chemical
Reg. No./Substance:
0/Glycoconjugates; 0/Receptors, Neurokinin-1; 0/Receptors, Neurotransmitter; 0/Tachykinins; 33507-63-0/Substance P

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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