| Substance P influenced gelatinolytic activity via reactive oxygen species in human pulp cells. | |
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MedLine Citation:
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PMID: 18822012 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To investigate the effects of substance P (SP) on gelatinolytic activity of matrix metalloproteinases (MMPs) in human pulp cells. METHODOLOGY: Human dental pulp cells were isolated and cultured. Subconfluent cells, between the third and sixth passages, were maintained under serum deprivation for 18 h followed by the treatment of varying doses of SP (1 pmol L(-1), 100 pmol L(-1), 10 nmol L(-1), 1 micromol L(-1) and 100 micromol L(-1)). Conditioned media were then subjected to gelatin zymography using 8% sodium dodecyl sulphate polyacrylamide gel electrophoresis minigels containing 1.5 g L(-1) gelatin. The effect of SP on intracellular reactive oxygen species (ROS) was also examined by confocal microscopy. ROS scavenger N-Acetyl-L-cysteine (NAC, 5 mmol L(-1)) was utilized to evaluate the roles of ROS pathway in mediating the impact of SP on cellular gelatinolytic activity. Data were analysed using analysis of variance with Bonferroni correction for multiple comparisons or an unpaired Student's t-test. RESULTS: Substance P, at levels above 1 micromol L(-1), remarkably enhanced MMP-2 activity reflected by the band migrating at 66 kDa (P < 0.05). A gelatinolytic band at approximately 44 kDa appeared to be intensified in a SP dose-dependent manner. In addition, it was demonstrated that SP could induce ROS production in pulp cells and ROS scavenger NAC was further found to significantly reduce MMP-2 activity (P < 0.05), as well as other bands of gelatinolytic proteinases. CONCLUSION: Substance P can influence gelatinolytic activity in human pulp cells via ROS pathway. |
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Authors:
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F-M Wang; T Hu; R Cheng; H Tan; X-D Zhou |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International endodontic journal Volume: 41 ISSN: 1365-2591 ISO Abbreviation: Int Endod J Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-09-29 Completed Date: 2009-01-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8004996 Medline TA: Int Endod J Country: England |
Other Details:
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Languages: eng Pagination: 856-62 Citation Subset: D |
Affiliation:
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State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcysteine
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pharmacology Cells, Cultured Culture Media, Conditioned Dental Pulp / drug effects*, enzymology Dose-Response Relationship, Drug Electrophoresis, Polyacrylamide Gel Free Radical Scavengers / pharmacology Gelatinases / administration & dosage, antagonists & inhibitors, pharmacology* Humans Matrix Metalloproteinase 2 / antagonists & inhibitors, drug effects Microscopy, Confocal Reactive Oxygen Species / antagonists & inhibitors, pharmacology* Substance P / administration & dosage, pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Conditioned; 0/Free Radical Scavengers; 0/Reactive Oxygen Species; 33507-63-0/Substance P; 616-91-1/Acetylcysteine; EC 3.4.24.-/Gelatinases; EC 3.4.24.24/MMP2 protein, human; EC 3.4.24.24/Matrix Metalloproteinase 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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