| Subpopulations of guinea-pig T lymphocytes defined by isoforms of the leucocyte common antigen. | |
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MedLine Citation:
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PMID: 1362184 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This report presents the characterization of three mouse monoclonal antibodies (mAb) reactive with the guinea-pig leucocyte common antigen (LCA); CD45 in the human nomenclature. One, IH-1, reacted with LCA on all leucocytes. The other two were more restricted: IH-2 recognized only the 220,000, 210,000 and 195,000 MW isoforms, and IH-4 the 220,000, 210,000 MW isoforms. Both IH-2 and IH-4 reacted with all B cells and all Kurloff cells [the putative guinea-pig natural killer (NK) cell]. IH-2, but not IH-4, reacted with monocytes and macrophages. Neither reacted with neutrophils. Most thymocytes expressed low levels of the IH-2 and IH-4 epitopes, with those expressing high levels located predominantly within the medulla. Most (90%) CD4+ T cells from newborn guinea-pigs expressed high levels of the IH-2 and IH-4 epitopes; this percentage decreased with age to 70% in 2-year-old animals. We have demonstrated that CD4+ T cells which express low levels of the IH-2 epitope also express low levels of the IH-4 epitope. CD8+ T cells can be divided into two subsets by IH-4 but not IH-2. The reactivities of IH-2 and IH-4 are remarkably similar to those of human anti-CD45RB and anti-CD45RA antibodies respectively. Analogies with man and other species suggest important functional differences for subpopulations of guinea-pig T lymphocytes defined by anti-CD45R antibodies. |
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Authors:
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I J Hart; H Schäfer; R J Scheper; G T Stevenson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Immunology Volume: 77 ISSN: 0019-2805 ISO Abbreviation: Immunology Publication Date: 1992 Nov |
Date Detail:
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Created Date: 1993-02-10 Completed Date: 1993-02-10 Revised Date: 2010-09-07 |
Medline Journal Info:
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Nlm Unique ID: 0374672 Medline TA: Immunology Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 377-84 Citation Subset: IM |
Affiliation:
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Lymphoma Research Unit, Tenovus Research Laboratory, University of Southampton, U.K. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aging
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immunology Animals Antibodies, Monoclonal / immunology Antigens, CD45 / analysis* B-Lymphocytes / immunology CD4-Positive T-Lymphocytes / immunology Electrophoresis, Polyacrylamide Gel Guinea Pigs Immunoenzyme Techniques Mice Mice, Inbred BALB C T-Lymphocyte Subsets / immunology* T-Lymphocytes / immunology Thymus Gland / immunology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; EC 3.1.3.48/Antigens, CD45 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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