Document Detail

Subpopulations of GFP-marked mouse pancreatic β-cells differ in size, granularity, and insulin secretion.
MedLine Citation:
PMID:  22919061     Owner:  NLM     Status:  MEDLINE    
There is growing information about the heterogeneity of pancreatic β-cells and how it relates to insulin secretion. This study used the approach of flow cytometry to sort and analyze β-cells from transgenic mice expressing green fluorescent protein (GFP) under the control of the mouse insulin I gene promoter. Three populations of β-cells with differing GFP brightness could be identified, which were classified as GFP-low, GFP-medium, and GFP-bright. The GFP-medium population comprised about 70% of the total. The GFP-low population had less insulin secretion as determined by the reverse hemolytic plaque assay and reduced insulin gene expression. Additionally, all three subpopulations of β-cells were found in mice of varying ages (embryonic d 15.5 and postnatal wk 1-9). The three populations from the youngest had larger cells (forward scatter) and less granularity (side scatter) than those from the adults. This approach opens up new ways to advance knowledge about β-cell heterogeneity.
Hitoshi Katsuta; Cristina Aguayo-Mazzucato; Rimiko Katsuta; Tomoyuki Akashi; Jennifer Hollister-Lock; Arun J Sharma; Susan Bonner-Weir; Gordon C Weir
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-08-23
Journal Detail:
Title:  Endocrinology     Volume:  153     ISSN:  1945-7170     ISO Abbreviation:  Endocrinology     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-22     Completed Date:  2013-01-17     Revised Date:  2013-11-05    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5180-7     Citation Subset:  AIM; IM    
Section on Islet Cell and Regenerative Biology, Research Division, Joslin Diabetes Center, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA.
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MeSH Terms
Cell Size*
Flow Cytometry
Green Fluorescent Proteins / genetics*
Insulin / secretion*
Insulin-Secreting Cells / cytology*,  metabolism,  secretion
Mice, Transgenic
Promoter Regions, Genetic
Grant Support
Reg. No./Substance:
0/Insulin; 147336-22-9/Green Fluorescent Proteins

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