| Subplasmalemmal microfilaments and microtubules in resting and phagocytizing cultivated macrophages. | |
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MedLine Citation:
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PMID: 4356569 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The subplasmalemmal organization of the free and glass-attached surfaces of resting and phagocytizing cultivated macrophages were examined in an attempt to define specific membrane-associated structures related to phagocytosis. From analysis of serial thin sections of oriented cells it was found that the subplasmalemmal region of the attached cell surface has a complex microfilament and microtubule organization relative to the subplasmalemmal area of the free surface. A filamentous network composed of 40-50-A microfilaments extended for a depth of 400-600 A from the attached plasma membrane. Immediately subjacent to the filamentous network was a zone of oriented bundles of 40-50-A microfilaments and a zone of microtubules. Additional microtubules were found to extend from the plasma membrane to the interior of the cell in close association with electron-dense, channellike structures. In contrast, the free aspect of the cultivated macrophage contained only the subplasmalemmal filamentous network. However, after a phagocytic pulse with polystyrene particles (14 microm diam) microtubules and oriented filaments similar to those found on the attached surface were observed surrounding the ingested particles. The observations reported in this paper provide support for the hypothesis that microfilaments and/or microtubules play a role in the translocation of plasma membrane required for the functionally similar processes of phagocytosis and cell attachment to glass. |
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Authors:
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E P Reaven; S G Axline |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of cell biology Volume: 59 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1973 Oct |
Date Detail:
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Created Date: 1974-01-15 Completed Date: 1974-01-15 Revised Date: 2010-06-22 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 12-27 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Membrane Cells, Cultured Inclusion Bodies* Macrophages / cytology* Male Mice Microscopy, Electron Microtubules* Peritoneal Cavity / cytology Phagocytosis* Polystyrenes |
| Chemical | |
Reg. No./Substance:
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0/Polystyrenes |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Copyright © 1973 by The Rockefeller University Press Received Day: 6 Month: 12 Year: 1972 Revision Received Day: 19 Month: 3 Year: 1973 Print publication date: Day: 1 Month: 10 Year: 1973 Volume: 59 Issue: 1 First Page: 12 Last Page: 27 ID: 2110909 PubMed Id: 4356569 |
| SUBPLASMALEMMAL MICROFILAMENTS AND MICROTUBULES IN RESTING AND PHAGOCYTIZING CULTIVATED MACROPHAGES | |
| Eve P. Reaven | |
| Stanton G. Axline | |
| From the Department of Medicine, Stanford University School of Medicine, and Stanford Medical Service, Veterans Administration Hospital, Palo Alto, California 94305 |
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