Document Detail

Submicrometer spatial resolution of metal-enhanced fluorescence.
MedLine Citation:
PMID:  14686781     Owner:  NLM     Status:  MEDLINE    
Enhanced fluorescence emission intensity from fluorescein was observed on glass slides covered with thin films of silver nanoparticles using a confocal laser-scanning microscope. The silver nanoparticle film increased the emission intensity of fluorescein by an average of at least three-fold in the area studied. Statistics are given on the enhancement of individual areas of the silver particle film with a resolution of approximately 210 nm. A histogram of intensity values indicates that the enhancement appears to occur without distinct subpopulations, with the exception that very high intensity subpopulations may occur but could not be resolved. Spatial features with dimensions near or smaller than the resolution limit of the confocal microscope, on the silver nanoparticle slide that enhanced the emission of fluorescein, were found using autocorrelation functions. These spatial features are of the same size as those found from the emission of slides containing silver nanoparticles only. These spatial features do not appear in control slides containing fluorescein without any silver nanoparticles. No long-range spatial ordering of the fluorescence enhancement on chemcially deposited silver nanoparticle slides was detected.
Vincent J Pugh; Henryk Szmacinski; Wayne E Moore; Chris D Geddes; Joseph R Lakowicz
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Applied spectroscopy     Volume:  57     ISSN:  0003-7028     ISO Abbreviation:  Appl Spectrosc     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-12-22     Completed Date:  2004-07-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372406     Medline TA:  Appl Spectrosc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1592-8     Citation Subset:  IM    
Microcosm, Inc., 9140 Guilford Rd., Suite O, Columbia, Maryland 21046-2585, USA.
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MeSH Terms
Fluorescein / chemistry
Metals / chemistry*
Microscopy, Confocal / methods*
Proteins / analysis,  chemistry
Sensitivity and Specificity
Serum Albumin, Bovine
Silver / chemistry
Grant Support
1 R3 CA097569-01/CA/NCI NIH HHS; RR-08119/RR/NCRR NIH HHS
Reg. No./Substance:
0/Metals; 0/Proteins; 0/Serum Albumin, Bovine; 1405-69-2/Avidin; 2321-07-5/Fluorescein; 58-85-5/Biotin; 7440-22-4/Silver

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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