Document Detail

Sublocalization of c-myb to 6q21----q23 by in situ hybridization and c-myb expression in a human teratocarcinoma with 6q rearrangements.
MedLine Citation:
PMID:  3007038     Owner:  NLM     Status:  MEDLINE    
We have sublocalized the human proto-oncogene c-myb by applying two different techniques: in situ hybridization of metaphase spreads and chromosome spot hybridization of flow-sorted chromosomes. For this we used a teratocarcinoma cell line carrying specific chromosome translocations involving the two chromosomes 6 and one chromosome 11. The distribution of the c-myb gene copies on the different translocation chromosomes revealed that c-myb is located in the region 6q21----q23. Because of the close proximity of the c-myb locus to the chromosomal breakpoints in the teratocarcinoma, we investigated whether c-myb was implicated in the development of this tumor. No rearrangement, deletion, or amplification of the gene was detected in the teratocarcinoma cells. Furthermore, the level of c-myb expression was comparable to that of other cell lines of nonhematopoietic origin. These results suggest that c-myb was not affected by the translocation and played no significant role in the development of this teratocarcinoma.
J W Janssen; P Vernole; P A de Boer; J W Oosterhuis; J G Collard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytogenetics and cell genetics     Volume:  41     ISSN:  0301-0171     ISO Abbreviation:  Cytogenet. Cell Genet.     Publication Date:  1986  
Date Detail:
Created Date:  1986-05-14     Completed Date:  1986-05-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0367735     Medline TA:  Cytogenet Cell Genet     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  129-35     Citation Subset:  IM    
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MeSH Terms
Cell Line
Chromosome Banding
Chromosome Mapping
Chromosomes, Human, 6-12 and X*
DNA Restriction Enzymes
DNA, Neoplasm / isolation & purification
Nucleic Acid Hybridization
RNA, Neoplasm / isolation & purification
Teratoma / genetics*
Reg. No./Substance:
0/DNA, Neoplasm; 0/RNA, Neoplasm; EC 3.1.21.-/DNA Restriction Enzymes

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