| Sublingual immunization protects against Helicobacter pylori infection and induces T and B cell responses in the stomach. | |
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MedLine Citation:
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PMID: 20696831 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Sublingual (SL) immunization has been described as an effective novel way to induce mucosal immune responses in the respiratory and genital tracts. We examined the potential of SL immunization against Helicobacter pylori to stimulate immune responses in the gastrointestinal mucosa and protect against H. pylori infection. Mice received two SL immunizations with H. pylori lysate antigens and cholera toxin as an adjuvant, and after challenge with live H. pylori bacteria, their immune responses and protection were evaluated, as were immune responses prior to challenge. SL immunization induced enhanced proliferative responses to H. pylori antigens in cervicomandibular lymph nodes and provided at least the same level of immune responses and protection as corresponding intragastric immunization. Protection in SL-immunized mice was associated with strong H. pylori-specific serum IgG and IgA antibody responses in the stomach and intestine, with strong proliferation and gamma interferon (IFN-γ) and interleukin-17 (IL-17) production by spleen and mesenteric lymph node T cells stimulated with H. pylori antigens in vitro, and with increased IFN-γ and IL-17 gene expression in the stomach compared to levels in infected unimmunized mice. Immunohistochemical studies showed enhanced infiltration of CD4(+) T cells and CD19(+) B cells into the H. pylori-infected stomach mucosa of SL-immunized but not unimmunized H. pylori-infected mice, which coincided with increased expression of the mucosal addressin cell adhesion molecule (MAdCAM-1) and T and B cell-attracting chemokines CXCL10 and CCL28. We conclude that, in mice, SL immunization can effectively induce protection against H. pylori infection in association with strong T and B cell infiltration into the stomach. |
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Authors:
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Sukanya Raghavan; Anna Karin Ostberg; Carl-Fredrik Flach; Annelie Ekman; Margareta Blomquist; Cecil Czerkinsky; Jan Holmgren |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-09 |
Journal Detail:
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Title: Infection and immunity Volume: 78 ISSN: 1098-5522 ISO Abbreviation: Infect. Immun. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-22 Completed Date: 2010-11-12 Revised Date: 2011-07-27 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: United States |
Other Details:
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Languages: eng Pagination: 4251-60 Citation Subset: IM |
Affiliation:
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University of Gothenburg Vaccine Research Institute, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden. sukanya.raghavan@microbio.gu.se |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Sublingual Animals B-Lymphocytes / physiology* Bacterial Vaccines / administration & dosage* Cell Adhesion Molecules / genetics, metabolism Female Gene Expression Regulation / physiology Helicobacter Infections / prevention & control* Helicobacter pylori / immunology* Lymph Nodes Mice Mice, Inbred C57BL Specific Pathogen-Free Organisms Stomach / cytology, immunology T-Lymphocytes / physiology* T-Lymphocytes, Helper-Inducer |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Vaccines; 0/Cell Adhesion Molecules; 0/Madcam1 protein, mouse |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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