| Sublethal damage during cryopreservation of rainbow trout sperm | |
| | |
MedLine Citation:
|
PMID: 9787069 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Although cellular damage during cryopreservation of freshwater fish spermatozoa has been reported in several studies, there is a lack of correlation between this damage and the fertility rates of eggs using postthawed milt. The apparent lack of such correlation may be due to other undetected sublethal cryodamage, which could affect the cell functionality and viability. This may be extremely important for freshwater fish spermatozoa whose ability to fertilize the egg requires dilution in water or hypoosmotic solutions, an hazardous environment for the cells. This study tested the change in cell permeability during cryopreservation, using Hoechst 33258 to assess cell permeability. The permeability of spermatozoa at different times after dilution in several hypoosmotic media were investigated. In the first trial, fresh semen, sperm diluted in freezing media (CPT), and freeze/thawed semen were studied. Three CPT were tested (Me2SO, DMA, and methanol). In the second trial, the addition of egg yolk as a membrane stabilizer was investigated. Samples were frozen at -20 degreesC/min in a programmable cooler and thawed in a 25 degreesC water bath. Dilution in the CPTs slightly increased the susceptibility of cells to damage but freezing/thawing caused a dramatic increase in the fragility of cells, which were killed in a few seconds after their contact with the hypoosmotic solutions. Egg yolk provided a significant protection to the membrane, allowing the cells a greater and more prolonged survival in the fertilization media. Samples frozen with Me2SO displayed the best results. These results are consistent with the achieved fertility rates that demonstrated sublethal cryodamage in the function of the sperm membrane that was not detected by standard procedures. Copyright 1998 Academic Press. |
| | |
Authors:
|
Cabrita; Alvarez; Anel; Rana; Herraez |
Related Documents
:
|
21110339 - Synthesis and in vitro anticancer activities of some selenadiazole derivatives. 15144499 - Cytotoxicity of lavender oil and its major components to human skin cells. 20542049 - Withanolides from withania aristata and their cytotoxic activity. 21504019 - Spatial and temporal dependence of the cerebral endothelial cells elasticity. 21183289 - A putatively functional haplotype in the gene encoding transforming growth factor beta-... 19524649 - Bc nanofibres: in vitro study of genotoxicity and cell proliferation. 23155249 - Suppression of cell invasion and migration by propofol are involved in down-regulating ... 22965909 - Crumbs limits oxidase-dependent signaling to maintain epithelial integrity and prevent ... 17381129 - Chemical caspase inhibitors enhance cell culture viabilities and protein titer. |
Publication Detail:
|
Type: JOURNAL ARTICLE |
Journal Detail:
|
Title: Cryobiology Volume: 37 ISSN: 1090-2392 ISO Abbreviation: Cryobiology Publication Date: 1998 Nov |
Date Detail:
|
Created Date: 1998-11-17 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0006252 Medline TA: Cryobiology Country: - |
Other Details:
|
Languages: Eng Pagination: 245-53 Citation Subset: - |
Affiliation:
|
Department of Biologia Celular y Anatomia, University of Leon, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Efficiency of trimetazidine in renal dysfunction secondary to cold ischemia-reperfusion injury: a pr...
Next Document: Spontaneous activity of preoptic neurons in slice preparations of the hypothalamus of European hamst...