| Subintimal Ki-67 as a synovial tissue biomarker for inflammatory arthropathies. | |
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MedLine Citation:
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PMID: 17613556 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: Ki-67 is expressed in the nuclei of dividing cells and can be used to assess proliferation of synovial inflammatory and stromal cells. We evaluated subintimal Ki-67+ cell density as a tissue biomarker for inflammatory arthropathies and compared it to subintimal CD68, a synovial biomarker of RA. METHODS: Subintimal Ki-67+ and CD68+ cell densities were measured immunohistochemically in synovial specimens obtained from patients with rheumatoid arthritis (RA; n = 19), osteoarthritis (OA; n = 18), "non-inflammatory" orthopaedic arthropathies (avascular necrosis, meniscus injury, femur fracture; n = 16), chronic septic arthritis (n = 9), and histologically normal synovium (n = 10). RESULTS: were correlated with a histological synovitis score. Utilising the areas under receiver operating characteristic curves (AUCs), we compared the abilities of Ki-67 and CD68 to differentiate among these arthropathies. Results: Ki-67 was expressed widely in the subintimal of inflamed specimens and in RA pannus invading hard tissues. Compared to normal controls, it was highly overexpressed in RA (26.6-fold) and chronic septic arthritis (55-fold), and mildly elevated in OA (3.9-fold) and orthopaedic arthropathies (2.1-fold). Ki-67 and CD68 differentiated similarly well between RA and OA (AUC: Ki-67 = 0.91, CD68 = 0.94), Ki-67 better between chronic septic arthritis and RA, and CD68 better between OA and normal controls. Ki-67 (r = 0.80) and CD68 (r = 0.79) correlated positively with the synovitis score. CONCLUSIONS: Subintimal Ki-67 was overexpressed in inflammatory arthropathies, distinguished among differentially inflamed arthropathies, and correlated positively with the histological severity of synovitis. It may prove useful in synovial tissue classification and as a synovial marker of disease activity in clinical trials when biopsies are available. |
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Authors:
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F Pessler; A Ogdie; C Diaz-Torne; L Dai; X Yu; E Einhorn; S Gay; H R Schumacher |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2007-07-05 |
Journal Detail:
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Title: Annals of the rheumatic diseases Volume: 67 ISSN: 1468-2060 ISO Abbreviation: Ann. Rheum. Dis. Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-01-14 Completed Date: 2008-02-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372355 Medline TA: Ann Rheum Dis Country: England |
Other Details:
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Languages: eng Pagination: 162-7 Citation Subset: IM |
Affiliation:
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The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA. frank.pessler@uniklinikum-dresden.de |
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD
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metabolism Antigens, Differentiation, Myelomonocytic / metabolism Arthritis, Rheumatoid / immunology, pathology* Cell Count / methods Cell Division Female Humans Ki-67 Antigen / metabolism* Knee Joint / immunology, pathology Male ROC Curve Synovial Membrane / immunology, pathology Synovitis / immunology, pathology* |
| Grant Support | |
ID/Acronym/Agency:
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T32-AR 007442/AR/NIAMS NIH HHS; T32-CA 09140/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Antigens, Differentiation, Myelomonocytic; 0/CD68 antigen, human; 0/Ki-67 Antigen |
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