Document Detail


Subdiaphragmatic murine electrophysiological studies: sequential determination of ventricular refractoriness and arrhythmia induction.
MedLine Citation:
PMID:  12750061     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Programmed electrical stimulation (PES) is a crucial aspect of the evaluation of the risk of arrhythmias in cardiac patients and provides a powerful tool for understanding the mechanisms of arrhythmia in experimental models. Whereas PES in the mouse is well characterized, the procedures allowing for follow-up studies in the same animal have not been developed. In this report, we describe a novel subdiaphragmatic approach that allows for repeat electrophysiological studies in the mouse. Under inhaled anesthesia, PES was performed in 36 wild-type mice via a stimulating electrode introduced through an epigastric incision and placed directly into the diaphragmatic surface of the heart. The procedure was repeated 7 days later. Ventricular effective refractory periods (VERP) did not change significantly between the initial and follow-up trials. Chronic treatment with amiodarone, however, was associated with a 70% prolongation in VERP from initial to follow-up studies (P < or = 0.001). In addition, PES of a genetically modified strain with sudden cardiac death, the connexin43 conditional knockout mouse consistently induced lethal polymorphic ventricular tachycardia. Thus sequential PES in mice is feasible with the use of a subdiaphragmatic approach, yields reproducible VERP values, and can be used to follow pharmacologically induced changes in VERP and identify mice at risk of lethal ventricular arrhythmias.
Authors:
David E Gutstein; Stephan B Danik; Jedd B Sereysky; Gregory E Morley; Glenn I Fishman
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2003-05-15
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  285     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-08-13     Completed Date:  2003-09-26     Revised Date:  2013-03-12    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H1091-6     Citation Subset:  IM    
Affiliation:
Department of Medicine, Division of Cardiology, New York University School of Medicine, VA Harbor Medical Center, 423 E. 23rd Street, 6 West-6005BW, New York, NY 10010, USA. david.gutstein@med.nyu.edu
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MeSH Terms
Descriptor/Qualifier:
Action Potentials / physiology*
Animals
Arrhythmias, Cardiac / diagnosis,  mortality,  physiopathology*
Connexin 43 / genetics
Death, Sudden, Cardiac
Diaphragm
Electric Stimulation / methods*
Electrocardiography
Heart / physiology*
Male
Mice
Mice, Inbred Strains
Mice, Mutant Strains
Pacemaker, Artificial
Predictive Value of Tests
Reproducibility of Results
Risk Factors
Ventricular Function
Grant Support
ID/Acronym/Agency:
HL 04222/HL/NHLBI NIH HHS; HL 30557/HL/NHLBI NIH HHS; HL 64757/HL/NHLBI NIH HHS; R01 HL064757/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Connexin 43

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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