Document Detail


Subcutaneous adipocytes promote the differentiation of squamous cell carcinoma cell line (DJM-1) in collagen gel matrix culture.
MedLine Citation:
PMID:  11511300     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cancer cell-stromal cell interaction plays a crucial role in the malignant growth of cancer cells. In the skin, the main stromal cell types consist of dermal fibroblasts and subcutaneous adipocytes. Fibroblasts are shown to promote the invasive growth of various cancer cell types. The interaction between cancer cells and stromal adipocytes, however, has not been sufficiently studied even in cutaneous carcinoma. To address the effects of adipocytes on the biologic behavior of cancer cells, we examined the growth and differentiation of a squamous cell carcinoma cell line of the skin (DJM-1), using a three-dimensional collagen gel matrix culture with a cutaneous environmental factor, air exposure. The growth was estimated by the uptake of bromodeoxy-uridine (BrdU) for 24 h. The BrdU indices of DJM-1 cells in stromal-cell-free, fibroblast-containing, and adipocyte- containing conditions were 19.7 +/- 1.9%, 19.8 +/- 2.8%, and 4.7 +/- 1.4%, respectively, whereas the BrdU index on the gel containing both fibroblasts and adipocytes was 10.4 +/- 3.3%. In terms of differentiation, DJM-1 cells cocultured with adipocytes constructed the best-organized stratified layer with a cornified-like structure in all conditions above. The differentiation markers involucrin and cytokeratin 10 were immunohistochemically detected in this structure of DJM-1 cells. Adipocyte-induced phenomena were not affected distinctively by air exposure. These results indicate that adipocytes, but not fibroblasts, promote the differentiation of squamous cell carcinoma cells (DJM-1) and inhibit their growth. These adipocyte-induced phenomena were not completely inhibited by fibroblasts. In conclusion, we suggest that stromal adipocytes may be involved in the differentiating mechanisms of cutaneous carcinoma cells.
Authors:
T Inoue; S Toda; Y Narisawa; H Sugihara
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  117     ISSN:  0022-202X     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-08-20     Completed Date:  2001-09-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  244-50     Citation Subset:  IM    
Affiliation:
Department of Pathology and Division of Dermatology, Saga Medical School, Saga, Japan. g9701@post.saga-med.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Adipocytes / cytology*,  physiology*
Animals
Carcinoma, Squamous Cell*
Cell Communication / physiology
Cell Culture Techniques / instrumentation,  methods
Cell Differentiation / drug effects,  physiology
Cell Division / drug effects,  physiology
Collagen / analysis,  pharmacology*
Extracellular Matrix / chemistry,  physiology
Gels
Humans
Insulin-Like Growth Factor II / pharmacology
Kidney / cytology
Leptin / pharmacology
Matrix Metalloproteinase 2 / analysis
Skin / cytology
Skin Neoplasms*
Stromal Cells / cytology,  physiology
Tumor Cells, Cultured / cytology
Tumor Necrosis Factor-alpha / pharmacology
Chemical
Reg. No./Substance:
0/Gels; 0/Leptin; 0/Tumor Necrosis Factor-alpha; 67763-97-7/Insulin-Like Growth Factor II; 9007-34-5/Collagen; EC 3.4.24.24/Matrix Metalloproteinase 2

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