| Subclinical scrapie infection in a resistant species: persistence, replication, and adaptation of infectivity during four passages. | |
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MedLine Citation:
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PMID: 12424693 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cross-species infection with transmissible spongiform encephalopathy agents may lead to subclinical infection and to adaptation of the infection to new species. This is of particular concern for the millions of people possibly exposed to bovine spongiform encephalopathy (BSE) by consumption of BSE-infected beef. Subclinical infection was studied by making 4 serial passages of hamster scrapie agent (263K) in mice. At each step, infectivity was followed by inoculation of hamsters and mice. Subclinical infection was demonstrated either by detection of abnormal protease-resistant prion protein (PrP-res) or in the absence of PrP-res by detection of infectivity. Replication and adaptation of hamster infectivity in mice was shown in year 2 after initial mouse passage. In third and fourth passages, dual-tropic, mouse-tropic, and hamster-tropic infectivity was found in different animals. In some cases infectivity similar to the original 263K hamster scrapie strain was found after 2 or 3 serial mouse passages totaling 1200-1550 days. |
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Authors:
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Richard Race; Kimberly Meade-White; Anne Raines; Gregory J Raymond; Byron Caughey; Bruce Chesebro |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of infectious diseases Volume: 186 Suppl 2 ISSN: 0022-1899 ISO Abbreviation: J. Infect. Dis. Publication Date: 2002 Dec |
Date Detail:
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Created Date: 2002-11-08 Completed Date: 2002-12-27 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0413675 Medline TA: J Infect Dis Country: United States |
Other Details:
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Languages: eng Pagination: S166-70 Citation Subset: AIM; IM |
Affiliation:
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Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, 903 S. Fourth Street, Hamilton, MT 59840, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological* Animals Brain Chemistry Carrier State Cricetinae Endopeptidases / metabolism Mice Mice, Inbred C57BL PrPSc Proteins / analysis, metabolism, pathogenicity* Scrapie / physiopathology*, virology Serial Passage Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/PrPSc Proteins; EC 3.4.-/Endopeptidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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