Document Detail


Subclassification of lymphoproliferative disorders in serous effusions: a 10-year experience.
MedLine Citation:
PMID:  23460311     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Rare studies have reported the application of multiple ancillary tests to the diagnosis of lymphoproliferative disorder in serous effusions. In the current study, the authors evaluated the effectiveness of using an algorithm for the triage of serous effusions and the contribution of ancillary studies to achieve a specific subtype of lymphoproliferative disorder.
METHODS: Serous effusion samples that had a final diagnosis of lymphoproliferative disorder or suspicious for lymphoma were selected from cases that were diagnosed between 2001 and 2010. Data were collected on patient and sample characteristics as well as results from immunophenotype and molecular studies.
RESULTS: In total, 168 serous effusions were identified from 110 patients. The most common site of involvement was the pleural cavity (n = 133) followed by the peritoneal cavity (n = 30) and pericardial cavity (n = 5). The volume of serous effusions ranged from 2 mL to 1000 mL (mean, 238 mL). In 42 patients (38.2%), serous effusions were the primary source of diagnosis. In 129 patients who had a diagnosis of LPD, either generic (n = 82) or specific (n = 47) ancillary tests were performed as a single test in 58 samples (67.4%) or as a combination of multiple studies in 19 samples (23.2%). Immunophenotyping was successful in almost all samples that had a specific subtype with 16 B-cell and 4 T-cell lymphomas being diagnosed. More samples with a specific subtype of lymphoma underwent molecular tests compared with those who had a generic diagnosis (19.1% vs 13.4%).
CONCLUSIONS: Successful, specific subtyping of lymphoproliferative disorders was achieved in approximately 33% of cases that were tested for ancillary studies following an approach for the triage and aliquoting of serous effusion samples.
Authors:
Leung Chu Tong; Hyang-Mi Ko; Mauro Ajaj Saieg; Scott Boerner; William R Geddie; Gilda da Cunha Santos
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer cytopathology     Volume:  121     ISSN:  1934-6638     ISO Abbreviation:  Cancer Cytopathol     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-06-27     Completed Date:  2013-07-12     Revised Date:  2013-07-24    
Medline Journal Info:
Nlm Unique ID:  101499453     Medline TA:  Cancer Cytopathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  261-70     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 American Cancer Society.
Affiliation:
Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Ascitic Fluid / metabolism,  pathology*
Cytodiagnosis / methods
Diagnosis, Differential
Female
Flow Cytometry
Humans
Immunohistochemistry
Immunophenotyping
In Situ Hybridization, Fluorescence
Lymphoma, B-Cell / classification,  diagnosis,  genetics
Lymphoma, T-Cell / classification,  diagnosis,  genetics
Lymphoproliferative Disorders / classification,  diagnosis*,  genetics
Male
Middle Aged
Pericardial Effusion / metabolism,  pathology*
Pleural Effusion / metabolism,  pathology*
Reproducibility of Results
Sensitivity and Specificity
Young Adult
Grant Support
ID/Acronym/Agency:
TGT-53 912//Canadian Institutes of Health Research

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